表面等离子体波导共振（PWR）光谱法用于直接观察G蛋白偶联受体（GPCR）/G蛋白相互作用的速率并定量亲和力。
Plasmon-waveguide resonance (PWR) spectroscopy for directly viewing rates of GPCR/G-protein interactions and quantifying affinities.
作者信息
Hruby Victor J, Tollin Gordon
机构信息
Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA.
出版信息
Curr Opin Pharmacol. 2007 Oct;7(5):507-14. doi: 10.1016/j.coph.2007.08.001. Epub 2007 Sep 14.
Abstract
Plasmon-waveguide resonance (PWR) spectroscopy is an optical technique that has been developed in our laboratories and applied to the study of membrane-associated proteins, especially GPCRs. It has high sensitivity and requires no labeling of materials, and it can monitor changes in proteolipid mass density and conformation in real time using plasmon excitation by light polarized both perpendicular and parallel to the resonator surface. Direct measurements will be described of the association of ligands and G-proteins to GPCRs incorporated into a self-assembled lipid bilayer deposited on the silica surface of a PWR resonator. These studies have provided new insights into the functioning of this important class of signaling proteins.
摘要
表面等离子体波导共振（PWR）光谱学是一种我们实验室开发的光学技术，已应用于膜相关蛋白的研究，尤其是G蛋白偶联受体（GPCRs）。它具有高灵敏度，无需对材料进行标记，并且可以通过与谐振器表面垂直和平行偏振的光进行表面等离子体激元激发，实时监测蛋白脂质质量密度和构象的变化。将描述对结合到沉积在PWR谐振器二氧化硅表面的自组装脂质双层中的GPCRs的配体和G蛋白结合的直接测量。这些研究为这类重要信号蛋白的功能提供了新的见解。
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