Jo Jun-ichiro, Tabata Yasuhiko
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto, Japan.
Eur J Pharm Biopharm. 2008 Jan;68(1):90-104. doi: 10.1016/j.ejpb.2007.04.021. Epub 2007 Jul 21.
The recent rapid progress of molecular biology together with the steady progress of genome projects has given us some essential and revolutionary information about DNA and RNA to elucidate various biological phenomena at a genetic level. Under these circumstances, the technology and methodology of gene transfection have become more and more important to enhance the efficacy of gene therapy for several diseases. In addition, gene transfection is a fundamental technology indispensable to the further research development of basic biology and medicine regarding stem cells. Stem cells genetically manipulated will enhance the therapeutic efficacy of cell transplantation. In this paper, the carrier and technology of gene delivery are briefly overviewed while the applications to the basic researches of biology and medicine as well as regenerative medical therapy are introduced. A new non-viral carrier and the cell culture system are described to efficiently manipulate stem cells.
近年来，分子生物学的迅速发展以及基因组计划的稳步推进，为我们提供了一些关于DNA和RNA的重要且具有革命性的信息，以便在基因层面阐明各种生物学现象。在这种情况下，基因转染技术和方法对于提高多种疾病的基因治疗效果变得越来越重要。此外，基因转染是基础生物学和医学关于干细胞进一步研究发展不可或缺的一项基础技术。经过基因操作的干细胞将提高细胞移植的治疗效果。本文简要概述了基因递送的载体和技术，同时介绍了其在生物学和医学基础研究以及再生医学治疗中的应用。还描述了一种新型非病毒载体和细胞培养系统，以有效操控干细胞。
