Le Goascogne C, Sananès N, Gouézou M, Takemori S, Kominami S, Baulieu E E, Robel P
INSERM U33, Communications Hormonales, Université Paris-sud, Bicêtre, France.
J Reprod Fertil. 1991 Nov;93(2):609-22. doi: 10.1530/jrf.0.0930609.
The cytochrome P-450(17 alpha)-hydroxylase, 17----20 lyase (P-450(17 alpha)) is the key enzyme responsible for the biosynthesis of androgens in steroidogenic organs. Its cellular localization has been examined with an immunohistochemical technique. In immature rat ovary, P-450(17 alpha) was first detected in sparse interstitial cells on postnatal Day 8. The number of immunoreactive interstitial cells increased thereafter and the intensity of P-450(17 alpha) staining in these cells was highest at 3 weeks of age. The intensity of staining then started to decline and was very faint at Day 35. From 6 weeks on, the distribution of immunoreactive P-450(17 alpha) was of the adult type: it was detected exclusively in the thecal cells of the large antral, preovulatory, follicles. P-450(17 alpha) was not detectable during pregnancy except on the day of parturition, when thecal cells were transiently immunoreactive. The staining had vanished 24 h after delivery. Human chorionic gonadotrophin (hCG), injected into immature females on Days 24 to 26, induced P-450(17 alpha) prematurely in thecal cells. When injected on Days 12 to 14 of pregnancy, hCG also induced P-450(17 alpha) in the thecal cells surrounding the largest follicles, whereas the interstitial and luteal cells were not immunostained. The antiprogestin RU486, injected on Day 16 of pregnancy, reinstated P-450(17 alpha) (and P-450scc) immunoreactivity in the thecal cells. Oestradiol selectively suppressed P-450(17 alpha) expression in the thecal cells of RU486-treated females. In immature guinea-pig ovary, P-450(17 alpha) was immunostained in thecal cells, not in interstitial cells, although the interstitial cells expressed the delta 5-3 beta-hydroxysteroid dehydrogenase. P-450(17 alpha) was also immunolocalized in the Leydig cells of rat and guinea-pig testes, and in the guinea-pig adrenal cortex (zonae fasciculata and reticularis), but not in the rat adrenal cortex. P-450(17 alpha) was not detectable in the brain of either rat or guinea-pig.
细胞色素P-450（17α）-羟化酶，17→20裂解酶（P-450（17α））是类固醇生成器官中雄激素生物合成的关键酶。已采用免疫组织化学技术检测其细胞定位。在未成熟大鼠卵巢中，出生后第8天首次在稀疏的间质细胞中检测到P-450（17α）。此后，免疫反应性间质细胞数量增加，这些细胞中P-450（17α）染色强度在3周龄时最高。然后染色强度开始下降，在第35天时非常微弱。从6周龄开始，免疫反应性P-450（17α）的分布为成年型：仅在大的有腔、排卵前卵泡的卵泡膜细胞中检测到。除分娩当天卵泡膜细胞短暂出现免疫反应性外，妊娠期间未检测到P-450（17α）。分娩后24小时染色消失。在出生后第24至26天给未成熟雌性大鼠注射人绒毛膜促性腺激素（hCG），可使卵泡膜细胞过早诱导产生P-450（17α）。在妊娠第12至14天注射hCG时，也可在最大卵泡周围的卵泡膜细胞中诱导产生P-450（17α），而间质细胞和黄体细胞未被免疫染色。在妊娠第16天注射抗孕激素RU486，可恢复卵泡膜细胞中P-450（17α）（和P-450scc）的免疫反应性。雌二醇选择性抑制RU486处理的雌性大鼠卵泡膜细胞中P-450（17α）的表达。在未成熟豚鼠卵巢中，P-450（17α）在卵泡膜细胞中被免疫染色，而不在间质细胞中，尽管间质细胞表达δ5-3β-羟类固醇脱氢酶。P-450（17α）也在大鼠和豚鼠睾丸的Leydig细胞以及豚鼠肾上腺皮质（束状带和网状带）中被免疫定位，但不在大鼠肾上腺皮质中。在大鼠或豚鼠的脑中未检测到P-450（17α）。
