Sasano H, Mason J I, Sasano N
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Mol Cell Endocrinol. 1989 Apr;62(2):197-202. doi: 10.1016/0303-7207(89)90006-3.
Cytochrome P-450 specific for steroid 17 alpha-hydroxylation (P-450(17 alpha] was immunohistochemically observed in pig adrenal cortex, testis and ovary by the biotin-streptavidin method using a specific antibody against P-450(17 alpha) purified from neonatal pig testis. In the adrenal cortex, P-450(17 alpha) was present in the zona fasciculata and reticularis while no immunoreactivity was observed in the zona glomerulosa, confirming the absence of 17-hydroxylated steroid synthesis in the zona glomerulosa. In the testis, P-450(17 alpha) was present exclusively in Leydig cells and immunoreactivity was absent in seminiferous tubules. In the ovary, immunoreactivity was observed only in the theca interna but not in the membrana granulosa of follicles. Among the tissues examined, the relative intensity of immunoreactivity was greatest in the Leydig cells, and progressively less in theca interna cells, outer fasciculata cells and inner fasciculata and reticularis cells.
采用从新生猪睾丸中纯化的针对甾体17α-羟化酶特异性细胞色素P-450（P-450（17α））的特异性抗体，通过生物素-链霉亲和素方法，在猪的肾上腺皮质、睾丸和卵巢中对甾体17α-羟化酶特异性细胞色素P-450（P-450（17α））进行了免疫组织化学观察。在肾上腺皮质中，P-450（17α）存在于束状带和网状带，而在球状带未观察到免疫反应性，证实球状带不存在17-羟化甾体合成。在睾丸中，P-450（17α）仅存在于睾丸间质细胞中，而在生精小管中不存在免疫反应性。在卵巢中，仅在卵泡膜内层观察到免疫反应性，而在卵泡颗粒膜中未观察到。在所检查的组织中，免疫反应性的相对强度在睾丸间质细胞中最大，在卵泡膜内层细胞、束状带外层细胞以及束状带内层和网状带细胞中逐渐降低。
