Smallshaw Joan E, Richardson James A, Vitetta Ellen S
The Cancer Immunobiology Center and the Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-8576, USA.
Vaccine. 2007 Oct 16;25(42):7459-69. doi: 10.1016/j.vaccine.2007.08.018. Epub 2007 Aug 30.
Ricin is a plant toxin that is a CDC level B biothreat. Our recombinant ricin A chain vaccine (RiVax), which contains mutations in both known toxic sites, has no residual toxicity at doses at least 800 times the immunogenic dose. RiVax without adjuvant given intramuscularly (i.m.) protected mice against intraperitoneally administered ricin. Furthermore the vaccine without alum was safe and immunogenic in human volunteers. Here we describe the development of gavage and aerosol ricin challenge models in mice and demonstrate that i.m. vaccination protects mice against ricin delivered by either route. Also RiVax protects against aerosol-induced lung damage as determined by histology and lung function tests.
蓖麻毒素是一种植物毒素，属于美国疾病控制与预防中心（CDC）的B级生物威胁物质。我们的重组蓖麻毒素A链疫苗（RiVax）在两个已知毒性位点均含有突变，在剂量至少为免疫原性剂量800倍时无残留毒性。肌肉注射（i.m.）无佐剂的RiVax可保护小鼠免受腹腔注射蓖麻毒素的侵害。此外，不含明矾的该疫苗在人类志愿者中是安全且具有免疫原性的。在此我们描述了小鼠灌胃和雾化蓖麻毒素攻击模型的建立，并证明肌肉注射疫苗可保护小鼠免受通过这两种途径递送的蓖麻毒素的侵害。此外，通过组织学和肺功能测试确定，RiVax还可预防雾化诱导的肺损伤。
