毛泡桐（玄参科）提取物的抗自由基活性。
Antiradical activity of Paulownia tomentosa (Scrophulariaceae) extracts.
作者信息
Smejkal Karel, Holubova Pavla, Zima Ales, Muselik Jan, Dvorska Margita
机构信息
Natural Drugs Department, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, CZ-612 42 Brno, Czech Republic.
出版信息
Molecules. 2007 Jun 27;12(6):1210-9. doi: 10.3390/12061210.
Paulownia tomentosa is a large indecidous tree planted mostly for its fast growing wood and decorative purposes. The tree is also used in traditional Chinese medicine. As a part of our study of natural polyphenols, the fruits of Paulownia tomentosa were extracted by EtOH and than subjected to liquid/liquid extraction. Fractions were analysed by TLC and HPLC to determine presence of phenolic substances. We identified and quantified acteoside (1) and isoacteoside (2) in the EtOAc and n-BuOH extracts; mimulone (3) and diplacone (4) in the MeOH extract. To determine the antiradical activity of extracts we used the anti DPPH and peroxynitrite assays. The activity was expressed as Trolox C equivalents, IC50 for DPPH scavenging and a time dependency course was established. The polyphenols content was determined; results were expressed as gallic acid equivalents. Using these methods we found the fractions of the n-BuOH, EtOAc and MeOH extracts that display antiradical activity, which could be exploited as potential pharmaceuticals.
毛泡桐是一种大型落叶乔木，主要因其速生木材和装饰用途而种植。这种树也用于传统中药。作为我们对天然多酚研究的一部分，毛泡桐果实用乙醇提取，然后进行液-液萃取。通过薄层色谱法（TLC）和高效液相色谱法（HPLC）分析各馏分，以确定酚类物质的存在。我们在乙酸乙酯和正丁醇提取物中鉴定并定量了毛蕊花糖苷（1）和异毛蕊花糖苷（2）；在甲醇提取物中鉴定并定量了刺槐苷（3）和二氢异黄酮（4）。为了测定提取物的抗自由基活性，我们采用了抗二苯基苦味酰基自由基（DPPH）和过氧亚硝酸盐测定法。活性以Trolox C当量表示，确定了DPPH清除的半数抑制浓度（IC50）并建立了时间依赖性过程。测定了多酚含量；结果以没食子酸当量表示。使用这些方法，我们发现了正丁醇、乙酸乙酯和甲醇提取物中具有抗自由基活性的馏分，这些馏分可作为潜在药物加以利用。
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