N-乙酰半胱氨酸减轻非酒精性脂肪性肝炎大鼠的氧化应激和肝脏病理变化。
N-acetylcysteine attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis.
作者信息
Thong-Ngam Duangporn, Samuhasaneeto Suchittra, Kulaputana Onanong, Klaikeaw Naruemon
机构信息
Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
World J Gastroenterol. 2007 Oct 14;13(38):5127-32. doi: 10.3748/wjg.v13.i38.5127.
AIM
To evaluate attenuating properties of N-acetylcysteine (NAC) on oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis (NASH).
METHODS
Male Sprague-Dawley rats were randomly divided into three groups. Group 1 (control, n=8) was free accessed to regular dry rat chow (RC) for 6 wk. Group 2 (NASH, n=8) was fed with 100% fat diet for 6 wk. Group 3 (NASH+NAC(20), n=9) was fed with 100% fat diet plus 20 mg/kg per day of NAC orally for 6 wk. All rats were sacrificed to collect blood and liver samples at the end of the study.
RESULTS
The levels of total glutathione (GSH) and hepatic malondialdehyde (MDA) were increased significantly in the NASH group as compared with the control group (GSH; 2066.7+/-93.2 vs 1337.5+/-31.5 micromol/L and MDA; 209.9+/-43.9 vs 3.8+/-1.7 micromol/g protein, respectively, P<0.05). Liver histopathology from group 2 showed moderate to severe macrovesicular steatosis, hepatocyte ballooning, and necroinflammation. NAC treatment improved the level of GSH (1394.8+/-81.2 micromol/L, P<0.05), it did not affect MDA (150.1+/-27.0 micromol/g protein), but led to a decrease in fat deposition and necroinflammation.
CONCLUSION
NAC treatment could attenuate oxidative stress and improve liver histology in rats with NASH.
目的
评估N-乙酰半胱氨酸（NAC）对非酒精性脂肪性肝炎（NASH）大鼠氧化应激和肝脏病理的减轻作用。
方法
雄性Sprague-Dawley大鼠随机分为三组。第1组（对照组，n = 8）自由进食常规干鼠粮（RC）6周。第2组（NASH组，n = 8）喂食100%脂肪饮食6周。第3组（NASH + NAC(20)组，n = 9）喂食100%脂肪饮食并每天口服20 mg/kg NAC 6周。研究结束时处死所有大鼠以采集血液和肝脏样本。
结果
与对照组相比，NASH组的总谷胱甘肽（GSH）水平和肝脏丙二醛（MDA）水平显著升高（GSH分别为2066.7±93.2 vs 1337.5±31.5 μmol/L，MDA分别为209.9±43.9 vs 3.8±1.7 μmol/g蛋白，P<0.05）。第2组的肝脏组织病理学显示中度至重度大泡性脂肪变性、肝细胞气球样变和坏死性炎症。NAC治疗提高了GSH水平（1394.8±81.2 μmol/L，P<0.05），对MDA无影响（150.1±27.0 μmol/g蛋白），但导致脂肪沉积和坏死性炎症减少。
结论
NAC治疗可减轻NASH大鼠的氧化应激并改善肝脏组织学。
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