Ozaras Resat, Tahan Veysel, Aydin Seval, Uzun Hafize, Kaya Safiye, Senturk Hakan
Altimermer Cad. 27/4, Kucukhamam, TR-34303 Fatih, Istanbul, Turkey.
World J Gastroenterol. 2003 Apr;9(4):791-4.
To investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol.
Twenty-four rats divided into three groups were fed with ethanol (6 g/kg/day, Group 1), ethanol and n-acetylcysteine (1 g/kg, Group 2), or isocaloric dextrose (control group, Group 3) for 4 weeks. Then animals were sacrificed under ether anesthesia, and intracardiac blood and liver tissues were obtained. Measurements were made in both serum and homogenized liver tissues. Malondialdehyde (MDA) level was measured by TBARS method. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels were studied by commercial kits. Kruskal-Wallis test was used for statistical analysis.
ALT and AST in Group 1 (154 U/L and 302 U/L, respectively) were higher than those in Group 2 (94 U/L and 155 U/L) and Group 3 (99 U/L and 168 U/L) (P=0.001 for both). Serum and tissue levels of MDA in Group 1 (1.84 nmol/mL and 96 nmol/100 mg-protein) were higher than that in Group 2 (0.91 nmol/mL and 64 nmol/100 mg protein) and Group 3 (0.94 nmol/mL and 49 nmol/100 mg-protein) (P<0.001 for both). On the other hand, serum GSH-Px level in Group 1 (8.21 U/g Hb) was lower than that in Group 2 (16 U/g Hb) and Group 3 (16 U/g-Hb) (P<0.001). Serum and liver tissue levels of SOD in Group 1 (11 U/mL and 26 U/100 mg-protein) were lower than that in Group 2 (18 U/mL and 60 U/100 mg protein) and Group 3 (20 U/mL and 60 U/100 mg-protein) (P<0.001 for both).
Ethanol-induced liver damage was associated with oxidative stress, and co-administration of n-acetylcysteine attenuates this damage effectively in rat model.
研究N-乙酰半胱氨酸对经口给予乙醇的大鼠的自由基清除作用。
将24只大鼠分为三组，分别给予乙醇（6克/千克/天，第1组）、乙醇和N-乙酰半胱氨酸（1克/千克，第2组）或等热量的葡萄糖（对照组，第3组），持续4周。然后在乙醚麻醉下处死动物，获取心脏血液和肝脏组织。对血清和肝脏匀浆组织进行检测。采用硫代巴比妥酸反应物（TBARS）法测定丙二醛（MDA）水平。使用商业试剂盒研究谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）水平。采用Kruskal-Wallis检验进行统计分析。
第1组的谷丙转氨酶（ALT）和谷草转氨酶（AST）（分别为154 U/L和302 U/L）高于第2组（94 U/L和155 U/L）和第3组（99 U/L和168 U/L）（两者P均=0.001）。第1组的血清和组织MDA水平（1.84纳摩尔/毫升和96纳摩尔/100毫克蛋白质）高于第2组（0.91纳摩尔/毫升和64纳摩尔/100毫克蛋白质）和第3组（0.94纳摩尔/毫升和49纳摩尔/100毫克蛋白质）（两者P<0.001）。另一方面，第1组的血清GSH-Px水平（8.21 U/克血红蛋白）低于第2组（16 U/克血红蛋白）和第3组（16 U/克血红蛋白）（P<0.001）。第1组的血清和肝脏组织SOD水平（11 U/毫升和26 U/100毫克蛋白质）低于第2组（18 U/毫升和60 U/100毫克蛋白质）和第3组（20 U/毫升和60 U/100毫克蛋白质）（两者P<0.001）。
乙醇诱导的肝损伤与氧化应激有关，在大鼠模型中，联合给予N-乙酰半胱氨酸可有效减轻这种损伤。
