Das Subir Kumar, Gupta Geetanjali, Rao D N, Vasudevan D M
Department of Biochemistry, Amrita Institute of Medical Sciences, Elamakkara P.O.. Cochin 682 026, India.
Indian J Exp Biol. 2007 Aug;45(8):683-8.
The alcoholic liver disease usually causes overall immunological alterations which might be attributed to hepatic disease, to ethanol action, and/or to malnourishment. In the present study, efficacy of lecithin with vitamin-B complex to treat ethanol induced immunomodulatory activity was compared with the effect of lecithin alone and tocopheryl acetate (vitamin E). Ethanol (1.6 g/kg body wt/day for 12 weeks) exposure increased thiobarbituric acid reactive substance (TBARS) level, while decreased superoxide dismutase (SOD) activity and reduced glutathione (GSH) content in whole blood hemolysate of 8-10 week-old male BALB/c mice (weighing 20-30 g). The activities of transaminase (AST and ALT) enzymes, interleukin (IL)-10 and gamma interferon (IFN-gamma) elevated, while IL-2 and IL-4 reduced in mice serum due to ethanol exposure. These suggested that oxidative stress and immunomodulatory activities were interdependent and associated with ethanol induced liver damage. Lecithin treatment significantly reduced AST (32.44%), ALT (32.09%), IL-10 (25.63%) activities and TBARS content (12.76%) compared to ethanol treated group. However, lecithin with vitamin-B complex treatment, significantly reduced AST (62.83%); ALT (61.96%); IL-10 (35.88%); IFN-gamma (22.55%) activities and TBARS content (31.58%), while significantly elevated GSH content (36.49%) and SOD activity (61.21%). Tocopheryl acetate treatment significantly reduced AST (62.83%); ALT (61.54%); IL-10 (36.35%): IFN-gamma (23.28%) activities and TBARS content (35.84%). while significantly elevated GSH content (28.76%) and SOD activity (62.42%) compared to ethanol treated group. These findings persuasively argued that lecithin with vitamin-B complex was a new promising therapeutic approach in controlling ethanol induced immunomodulatory activities involving liver damage processes. Prevention of oxidative stress with correction of nutritional deficiency caused alteration in the ethanol-induced immunomodulatory activities and associated liver diseases.
酒精性肝病通常会导致整体免疫改变，这可能归因于肝脏疾病、乙醇作用和/或营养不良。在本研究中，将含有复合维生素B的卵磷脂治疗乙醇诱导的免疫调节活性的效果，与单独使用卵磷脂以及生育酚醋酸酯（维生素E）的效果进行了比较。给8至10周龄雄性BALB/c小鼠（体重20至30克）每天经口给予乙醇（1.6克/千克体重，持续12周），可使全血溶血产物中的硫代巴比妥酸反应性物质（TBARS）水平升高，同时超氧化物歧化酶（SOD）活性降低，还原型谷胱甘肽（GSH）含量减少。由于乙醇暴露，小鼠血清中的转氨酶（AST和ALT）、白细胞介素（IL）-10和γ干扰素（IFN-γ）活性升高，而IL-2和IL-4活性降低。这些结果表明，氧化应激和免疫调节活性相互依存，并与乙醇诱导的肝损伤有关。与乙醇处理组相比，卵磷脂处理显著降低了AST（32.44%）、ALT（32.09%）、IL-10（25.63%）活性以及TBARS含量（12.76%）。然而，含有复合维生素B的卵磷脂处理显著降低了AST（62.83%）、ALT（61.96%）、IL-10（35.88%）、IFN-γ（2%）活性以及TBARS含量（31.58%），同时显著提高了GSH含量（36.49%）和SOD活性（61.21%）。与乙醇处理组相比，生育酚醋酸酯处理显著降低了AST（62.83%）、ALT（61.54%）、IL-10（36.35%）、IFN-γ（23.28%）活性以及TBARS含量（35.84%），同时显著提高了GSH含量（28.76%）和SOD活性（62.42%）。这些发现有力地表明，含有复合维生素B的卵磷脂是控制乙醇诱导的涉及肝损伤过程的免疫调节活性的一种新的有前景的治疗方法。通过纠正营养缺乏来预防氧化应激，可改变乙醇诱导的免疫调节活性及相关肝脏疾病。
