[鼠疫监测项目中基因与抗原检测方法的应用及评价研究]
[Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program].
作者信息
Hai Rong, Yu Dong-zheng, Shi Xian-ming, Zhang Zhong-bing, Tang Yong-jiao, Wang Peng, Xia Lian-xu, Wei Shao-zhen, Xu Bing, Qin Ying-xu, Zhang Zhi-kai, Shi Guo-xiang, Cai Hong, Zhang En-min, Wei Jian-chun, Geng Ying-zhi, Huang De-hui, Zhao Bin, Wang Li-mao, Ma Feng-qin, Huang Fu, Wang Yue, Zhang Tao, Zhang Jian-hua
机构信息
State Kay Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 May;28(5):426-9.
OBJECTIVE
To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
METHODS
1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
RESULTS
The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
CONCLUSION
The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
目的
在鼠疫监测项目中应用并评估特定基因和抗原检测的新方法。
方法
采用内部质量控制多重聚合酶链反应、免疫层析法标记的F1抗原以及酶联免疫吸附测定法，对来自鼠疫自然疫源地的1798份样本进行检测。鼠疫耶尔森菌培养及反向间接血凝试验用作参考诊断方法。
结果
鼠疫耶尔森菌培养以及基因和抗原检测的总体阳性率为7.34%，与单独使用鼠疫耶尔森菌培养方法的6.28%相比，提高了16.81%。符合率为97.13%。
结论
所采用的特定基因和抗原检测新标准可提高鼠疫诊断的阳性率。
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