Lan Quan-xue, Hu Qing-hua, Shi Xiao-lu, Wang Bing, Lin Yi-man, Cheng Jin-quan, Zhang Shun-xiang
Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2008 May;42(5):317-20.
To analyze the genetic relations of Shigella isolated from Shenzhen in 2001-2006 and develop primary molecular subtyping surveillance network of Shigella.
Chromosomal DNAs from 55 isolated in agarose were digested with the restriction enzyme Xba I, and then were analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns were clustered using BioNumerics software.
All 41 distinctive PFGE patterns were identified among 55 strains. 32 strains belonged to one cluster. Differences were observed in other strains.
Both genetic-related clones and non-related clones of Shigella existed in Shenzhen. The development of PFGE molecular subtyping surveillance network would contribute to the active surveillance, outbreak investigation and source tracking for Shigellosis.
分析2001 - 2006年从深圳分离的志贺菌的遗传关系，建立志贺菌分子分型初步监测网络。
用限制性内切酶Xba I消化琼脂糖中分离的55株菌的染色体DNA，然后用脉冲场凝胶电泳进行分析。使用BioNumerics软件对脉冲场凝胶电泳（PFGE）图谱进行聚类分析。
55株菌株共鉴定出41种不同的PFGE图谱。32株属于一个聚类。其他菌株存在差异。
深圳存在遗传相关和非相关的志贺菌克隆。PFGE分子分型监测网络的建立将有助于志贺菌病的主动监测、暴发调查和溯源追踪。
