Zhang Lijuan, Jin Zhang, Xia Shengli, Zhang Jingshan, Li Menglei, Fu Xiuping, Luan Mingchun
Department of Rickettsiology, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Southeast Asian J Trop Med Public Health. 2007 May;38(3):482-6.
To obtain knowledge of the genetic characteristics and types of the epidemic strains of Orientia tsutsugamushi in the first outbreak of scrub typhus in Henan Province, genus and type-specific primers were employed to amplify a fragment of the gene of 56 kDa protein. Serotyping demonstrated that, of the 19 patients [15 patients in recovery phase (10-40 days) and 4 of patients in acute phase (1-7 days)], 4 were infected with Gilliam type, 8 with Kato type, 6 with Karp type, and 1 with an unknown type. Successful genotyping was obtained for only 3 patients, indicating that 2 were infected with Karp type and 1 with Taiwan Kato type. Thus the outbreak of scrub typhus in Henan Province was caused by at least two epidemic strains.
为了解河南省首次恙虫病暴发中恙虫病东方体流行株的基因特征和类型，采用属特异性和型特异性引物扩增56 kDa蛋白基因片段。血清学分型显示，19例患者[15例处于恢复期（10 - 40天），4例处于急性期（1 - 7天）]中，4例感染Gilliam型，8例感染Kato型，6例感染Karp型，1例感染类型不明。仅3例患者成功进行了基因分型，表明2例感染Karp型，1例感染台湾Kato型。因此，河南省恙虫病暴发至少由两种流行株引起。
