Serichantalergs Oralak, Jensen L B, Pitarangsi C, Mason C J, Dalsgaard A
Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand.
Southeast Asian J Trop Med Public Health. 2007 May;38(3):501-6.
A total of 495 Campylobacterjejuni and 122 C. coli isolated from Thai children were screened for macrolide (erythromycin and azithromycin) resistance by disk diffusion assay. Minimum inhibitory concentrations for erythromycin, azithromycin, nalidixic acid, ciprofloxacin, tetracycline, streptomycin, gentamicin and chloramphenicol were further determined for these macrolide-resistant Campylobacter isolates. Presence of known point mutations resulting in reduced susceptibility to macrolides was investigated by PCR and DNA sequencing. Seventeen percent (23/122) of C. coli and 2.4% (12/495) of C. jejuni isolates were resistant to macrolides. By sequencing domain V of the 23S ribosomal DNA from all 35 macrolide-resistant isolates, a known point mutation of 23S rRNA associated with reduced susceptibility to macrolides was detected in all isolates except one. Among the macrolide-resistant isolates, all were multiply resistant to nalidixic acid and ciprofloxacin, of which the latter is the preferred antimicrobial used for diarrheal treatment in Thailand. Furthermore, most macrolide-resistant isolates were also resistant to tetracycline and streptomycin. The spread of macrolide and quinolone resistant Campylobacter should be monitored closely in Thailand and elsewhere as these antimicrobials are preferred drugs for treatment of diarrhea.
通过纸片扩散法对从泰国儿童中分离出的495株空肠弯曲菌和122株结肠弯曲菌进行大环内酯类（红霉素和阿奇霉素）耐药性筛查。对这些耐大环内酯类弯曲菌分离株进一步测定了红霉素、阿奇霉素、萘啶酸、环丙沙星、四环素、链霉素、庆大霉素和氯霉素的最低抑菌浓度。通过聚合酶链反应（PCR）和DNA测序研究导致对大环内酯类敏感性降低的已知点突变的存在情况。结肠弯曲菌分离株中有17%（23/122）、空肠弯曲菌分离株中有2.4%（12/495）对大环内酯类耐药。通过对所有35株耐大环内酯类分离株的23S核糖体DNA的V结构域进行测序，除一株外，在所有分离株中均检测到与大环内酯类敏感性降低相关的23S rRNA已知点突变。在耐大环内酯类分离株中，所有菌株对萘啶酸和环丙沙星均多重耐药，其中环丙沙星是泰国治疗腹泻的首选抗菌药物。此外，大多数耐大环内酯类分离株对四环素和链霉素也耐药。在泰国和其他地区，应密切监测耐大环内酯类和喹诺酮类弯曲菌的传播情况，因为这些抗菌药物是治疗腹泻的首选药物。
