Jones Jeryl C, Appt Susan E, Bourland J Daniel, Hoyer Patricia B, Clarkson Thomas B, Kaplan Jay R
Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic and State University, Blacksburg, VA, USA.
J Am Assoc Lab Anim Sci. 2007 Sep;46(5):54-63.
Macaques are important models for menopause and associated diseases in women. A sensitive, noninvasive technique for quantifying changes in ovarian morphology would facilitate longitudinal studies focused on the health-related sequelae of naturally occurring or experimentally induced alterations in ovarian structure and function. Multidetector computed tomography (MDCT) is a fast, non-invasive imaging technique that uses X-rays, multiple rows of detectors, and computers to generate detailed slice images of structures. The purpose of this study was to describe the utility of MDCT for reliably characterizing ovarian morphology in macaques. Five macaques were scanned using contrast-enhanced MDCT. The following characteristics were described: 1) appearance of ovaries and adjacent landmarks, 2) effects of varying technical protocols on ovarian image quality, 3) radiation doses delivered to the pelvic region during scanning, and 4) MDCT estimates of ovarian volume and antral follicle counts versus those measured directly in ovarian tissue. Ovaries were distinguishable in all MDCT scans and exhibited heterogeneous contrast enhancement. Antral follicles appeared as focal areas of nonenhancement. Ovarian image quality with 5 pediatric scanning protocols was sufficient for discriminating ovarian margins. Pelvic region radiation doses ranged from 0.5 to 0.7 rad. Antral follicles counted using MDCT ranged from 3 to 5 compared with 3 to 4 counted using histology. Ovarian volumes measured using MDCT ranged from 0.41 to 0.67 ml compared with 0.40 to 0.65 ml by water displacement. MDCT is a promising technique for measuring longitudinal changes in macaque ovarian morphology reliably and noninvasively.
猕猴是研究女性更年期及相关疾病的重要模型。一种用于量化卵巢形态变化的灵敏、非侵入性技术将有助于开展纵向研究，聚焦于卵巢结构和功能自然发生或实验诱导改变所带来的与健康相关的后遗症。多排探测器计算机断层扫描（MDCT）是一种快速、非侵入性的成像技术，它利用X射线、多排探测器和计算机生成结构的详细切片图像。本研究的目的是描述MDCT在可靠表征猕猴卵巢形态方面的效用。使用对比增强MDCT对5只猕猴进行扫描。描述了以下特征：1）卵巢及相邻标志物的外观，2）不同技术方案对卵巢图像质量的影响，3）扫描期间盆腔区域的辐射剂量，4）MDCT对卵巢体积和窦卵泡计数的估计与直接在卵巢组织中测量的结果对比。在所有MDCT扫描中卵巢均可区分，且呈现出不均匀的对比增强。窦卵泡表现为无增强的局灶区域。5种儿科扫描方案下的卵巢图像质量足以区分卵巢边界。盆腔区域辐射剂量范围为0.5至0.7拉德。MDCT计数的窦卵泡数量为3至5个，而组织学计数为3至4个。MDCT测量的卵巢体积范围为0.41至0.67毫升，而通过排水法测量为0.40至0.65毫升。MDCT是一种有前景的技术，可可靠且非侵入性地测量猕猴卵巢形态的纵向变化。
