Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia, USA.
Am J Primatol. 2010 Jun;72(6):530-8. doi: 10.1002/ajp.20807.
The purpose of this study was to validate low radiation dose, contrast-enhanced, multi-detector computed tomography (MDCT) as a non-invasive method for measuring ovarian volume in macaques. Computed tomography scans of four known-volume phantoms and nine mature female cynomolgus macaques were acquired using a previously described, low radiation dose scanning protocol, intravenous contrast enhancement, and a 32-slice MDCT scanner. Immediately following MDCT, ovaries were surgically removed and the ovarian weights were measured. The ovarian volumes were determined using water displacement. A veterinary radiologist who was unaware of actual volumes measured ovarian CT volumes three times, using a laptop computer, pen display tablet, hand-traced regions of interest, and free image analysis software. A statistician selected and performed all tests comparing the actual and CT data. Ovaries were successfully located in all MDCT scans. The iliac arteries and veins, uterus, fallopian tubes, cervix, ureters, urinary bladder, rectum, and colon were also consistently visualized. Large antral follicles were detected in six ovaries. Phantom mean CT volume was 0.702+/-SD 0.504 cc and the mean actual volume was 0.743+/-SD 0.526 cc. Ovary mean CT volume was 0.258+/-SD 0.159 cc and mean water displacement volume was 0.257+/-SD 0.145 cc. For phantoms, the mean coefficient of variation for CT volumes was 2.5%. For ovaries, the least squares mean coefficient of variation for CT volumes was 5.4%. The ovarian CT volume was significantly associated with actual ovarian volume (ICC coefficient 0.79, regression coefficient 0.5, P=0.0006) and the actual ovarian weight (ICC coefficient 0.62, regression coefficient 0.6, P=0.015). There was no association between the CT volume accuracy and mean ovarian CT density (degree of intravenous contrast enhancement), and there was no proportional or fixed bias in the CT volume measurements. Findings from this study indicate that MDCT is a valid non-invasive technique for measuring the ovarian volume in macaques.
本研究的目的是验证低辐射剂量、对比增强、多探测器计算机断层扫描（MDCT）作为一种非侵入性方法，用于测量猕猴的卵巢体积。使用先前描述的低辐射剂量扫描方案、静脉内对比增强和 32 层 MDCT 扫描仪，对四个已知体积的体模和九只成熟雌性食蟹猴进行 CT 扫描。在 MDCT 后立即进行手术切除卵巢，并测量卵巢重量。使用水置换法确定卵巢体积。一位不知道实际体积的兽医放射科医生在笔记本电脑、手写笔显示器、手动勾画感兴趣区域和免费图像分析软件上，使用 MDCT 三次测量卵巢 CT 体积。一位统计学家选择并执行了所有比较实际数据和 CT 数据的测试。在所有 MDCT 扫描中均成功定位了卵巢。髂动脉和静脉、子宫、输卵管、宫颈、输尿管、膀胱、直肠和结肠也被一致地可视化。在六个卵巢中检测到大的窦卵泡。体模的平均 CT 体积为 0.702+/-SD 0.504 cc，平均实际体积为 0.743+/-SD 0.526 cc。卵巢的平均 CT 体积为 0.258+/-SD 0.159 cc，平均水置换体积为 0.257+/-SD 0.145 cc。对于体模，CT 体积的平均变异系数为 2.5%。对于卵巢，CT 体积的最小二乘平均变异系数为 5.4%。卵巢 CT 体积与实际卵巢体积显著相关（ICC 系数 0.79，回归系数 0.5，P=0.0006）和实际卵巢重量（ICC 系数 0.62，回归系数 0.6，P=0.015）。CT 体积的准确性与卵巢 CT 密度（静脉内对比增强程度）之间没有相关性，并且 CT 体积测量没有比例或固定偏差。本研究的结果表明，MDCT 是一种有效的非侵入性技术，可用于测量猕猴的卵巢体积。
