Fecht S, Wöhlke A, Hamann H, Distl O
Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559 Hannover, Germany.
J Vet Med A Physiol Pathol Clin Med. 2007 Oct;54(8):401-5. doi: 10.1111/j.1439-0442.2007.00966.x.
A deletion mutation in the canine multidrug resistance gene, MDR1, is associated with drug sensitivity. This was shown for several purebred dog breeds from the Collie lineage such as the Collie (rough-coated and smooth-coated), the Australian Shepherd and the Old English sheepdog. To determine whether the mdr1-1Delta mutation could be found in the newly bred German dog breed Elo which is based amongst other breeds on Old English sheepdogs, 177 blood samples representative for the Elo breed were collected. After DNA extraction, a polymerase chain reaction-based method with subsequent polyacrylamide gel electrophoresis was used for detection of the mdr1-1Delta mutation. The mdr1-1Delta allele was not observed in the Elos investigated. The probability that the mdr1-1Delta allele originated in the Old English sheepdog breed is segregating in the Elo population was estimated at 3.68 x 10(-17).
犬多药耐药基因MDR1中的缺失突变与药物敏感性相关。对于几个来自柯利犬系的纯种犬品种，如柯利犬（粗毛和光毛）、澳大利亚牧羊犬和古代英国牧羊犬，情况就是如此。为了确定在新培育的德国犬种埃洛犬（Elo）中是否能发现mdr1-1Delta突变，该犬种在其他品种中以古代英国牧羊犬为基础，收集了177份代表埃洛犬种的血样。DNA提取后，采用基于聚合酶链反应并随后进行聚丙烯酰胺凝胶电泳的方法检测mdr1-1Delta突变。在所研究的埃洛犬中未观察到mdr1-1Delta等位基因。估计mdr1-1Delta等位基因起源于古代英国牧羊犬品种并在埃洛犬群体中分离的概率为3.68×10^(-17)。
