Paciello O, Borzacchiello G, Varricchio E, Papparella S
Division of Pathology, Department of Pathology and Animal health, Faculty of Veterinary Medicine, University of Naples FEDERICO II, Via Delpino, 1, 80137 Naples, Italy.
J Vet Med A Physiol Pathol Clin Med. 2007 Oct;54(8):406-10. doi: 10.1111/j.1439-0442.2007.00961.x.
Peroxisome proliferator-activated receptor gamma (PPAR-gamma) is a ligand-activated transcriptional factor belonging to the steroid receptor superfamily. PPAR-gamma is expressed in multiple normal and neoplastic tissues, such as the breast, colon, lung, ovary and placenta. In addition to adipogenic and anti-inflammatory effects, PPAR-gamma activation has been shown to be anti-proliferative by its differentiation-promoting effect, suggesting that activation of PPAR-gamma may be useful in slowing or arresting the proliferation of de-differentiated tumour cells. In this study, we investigated the expression of PPAR-gamma in normal and neoplastic canine nasal epithelium. Twenty-five samples composed of five normal nasal epithelia and 20 canine nasal carcinomas, were immunohistochemically stained for PPAR-gamma. The specificity of the antibody was verified by Western Blot analysis. Confocal laser scanning microscopical investigation was also performed. In normal epithelium, the staining pattern was cytoplasmic and polarized at the cellular free edge. In carcinomas, the neoplastic cells showed mainly strong cytoplasmatic PPAR-gamma expression; moreover, perinuclear immunoreactivity was also detected and few neoplastic cells exhibited a nuclear positivity. Our results demonstrate different patterns of PPAR-gamma expression in normal canine nasal epithelium when compared with canine nasal carcinoma. The importance of this transcription factor in the pathophysiology of several different tumours has stimulated much research in this field and has opened new opportunities for the treatment of the tumours.
过氧化物酶体增殖物激活受体γ（PPAR-γ）是一种属于类固醇受体超家族的配体激活转录因子。PPAR-γ在多种正常组织和肿瘤组织中表达，如乳腺、结肠、肺、卵巢和胎盘。除了具有促脂肪生成和抗炎作用外，PPAR-γ激活还通过其促进分化的作用表现出抗增殖特性，这表明激活PPAR-γ可能有助于减缓或阻止去分化肿瘤细胞的增殖。在本研究中，我们调查了PPAR-γ在正常和肿瘤性犬鼻上皮中的表达情况。对由5个正常鼻上皮和20个犬鼻癌组成的25个样本进行了PPAR-γ免疫组织化学染色。通过蛋白质印迹分析验证了抗体的特异性。还进行了共聚焦激光扫描显微镜研究。在正常上皮中，染色模式为细胞质染色，且在细胞游离边缘呈极化分布。在癌组织中，肿瘤细胞主要表现为强烈的细胞质PPAR-γ表达；此外，还检测到核周免疫反应性，少数肿瘤细胞呈现核阳性。我们的结果表明，与犬鼻癌相比，正常犬鼻上皮中PPAR-γ的表达模式不同。这种转录因子在几种不同肿瘤的病理生理学中的重要性激发了该领域的大量研究，并为肿瘤治疗开辟了新的机会。
