Gianakopoulos G J, Prior R B
Dept of Internal Medicine, Ohio State University College of Medicine, Columbus 43210.
Med Lab Sci. 1991 Jul;48(3):183-8.
Chlamydia trachomatis produces small amounts of an endotoxin-like material. The Limulus amoebocyte lysate assay was used to evaluate chlamydial cell cultures and also the exudates from adult male patients with non-gonococcal urethritis, as a possible method to subdivide this condition into chlamydial and nonchlamydial urethritis. In vitro endotoxin assays were conducted in McCoy cell media using the Limulus assay, and endotoxin levels were consistently 10-fold less at 24 h than at 0, 48, 72, and 96 h, which may be accounted for by the unique growth cycle of chlamydia. In 75 males with non-gonococcal urethritis, urethral exudates were collected, serially diluted and assayed for endotoxin content. Of these, 27 (36%) had positive chlamydial cultures and 48 were negative. There was no statistically significant correlation between the level of endotoxin present and a positive or a negative culture for C. trachomatis (P greater than 0.05). Sensitivity and specificity of the assay were only 59% and 56%, respectively, at a 1 in 8 dilution; it was not useful in predicting chlamydial culture results in male patients with non-gonococcal urethritis.
沙眼衣原体产生少量类内毒素物质。采用鲎试剂法评估衣原体细胞培养物以及成年男性非淋菌性尿道炎患者的渗出物，作为将该疾病细分为衣原体性尿道炎和非衣原体性尿道炎的一种可能方法。使用鲎试剂法在 McCoy 细胞培养基中进行体外内毒素检测，内毒素水平在 24 小时时始终比 0、48、72 和 96 小时时低 10 倍，这可能是由衣原体独特的生长周期所致。在 75 名非淋菌性尿道炎男性患者中，收集尿道渗出物，进行系列稀释并检测内毒素含量。其中，27 例（36%）衣原体培养阳性，48 例阴性。所检测到的内毒素水平与沙眼衣原体培养阳性或阴性之间无统计学显著相关性（P＞0.05）。在 1:8 稀释度时，该检测方法的敏感性和特异性分别仅为 59%和 56%；在预测男性非淋菌性尿道炎患者的衣原体培养结果方面并无用处。
