通过扩增片段长度多态性（AFLP）分析确定泰国蓝蟹（远海梭子蟹）的遗传异质性。
Genetic heterogeneity of the blue swimming crab (Portunus pelagicus) in Thailand determined by AFLP analysis.
作者信息
Klinbunga Sirawut, Khetpu Kannika, Khamnamtong Bavornlak, Menasveta Piamsak
机构信息
Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong 1, Klong Luang, Pathum Thani, 12120, Thailand.
出版信息
Biochem Genet. 2007 Oct;45(9-10):725-36. doi: 10.1007/s10528-007-9110-1. Epub 2007 Sep 19.
Genetic diversity and population differentiation of the blue swimming crab (Portunus pelagicus) in Thailand, originating from Ranong and Krabi located in the Andaman Sea (west) and Chanthaburi, Prachuap Khiri Khan, and Suratthani located in the Gulf of Thailand (east), were examined by AFLP analysis. High genetic diversity of P. pelagicus in Thai waters was found (N=72). The four primer combinations generated 227 AFLP fragments, and the percentage of polymorphic bands in each geographic sample was 66.19-94.38%. The mean genetic distance between pairs of samples was 0.1151-0.2440. Geographic heterogeneity analyses using the exact test and FST-based statistics between all pairwise comparisons were statistically significant (P<0.01), indicating a fine-scale level of intraspecific population differentiation of Thai P. pelagicus. The estimated number of migrants per generation (Nem) was 0.26-0.76, suggesting restricted gene flow levels of P. pelagicus in Thai waters.
通过扩增片段长度多态性（AFLP）分析，对源自泰国安达曼海（西部）的拉廊和甲米以及泰国湾（东部）的尖竹汶、巴蜀和素叻他尼的蓝蟹（远海梭子蟹）的遗传多样性和种群分化进行了研究。研究发现泰国海域的远海梭子蟹具有较高的遗传多样性（N = 72）。四种引物组合产生了227个AFLP片段，每个地理样本中的多态性条带百分比为66.19 - 94.38%。样本对之间的平均遗传距离为0.1151 - 0.2440。使用精确检验和基于FST的统计方法对所有两两比较进行的地理异质性分析具有统计学意义（P < 0.01），表明泰国远海梭子蟹存在种内种群分化的精细尺度水平。每代迁移个体数（Nem）估计为0.26 - 0.76，表明泰国海域远海梭子蟹的基因流动水平受限。
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