Sun Chen, Xie Yuchun, Tian Qinglai, Liu Huizhou
Chemistry and Chemical Engineering Department, Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Phytochem Anal. 2008 Mar-Apr;19(2):160-3. doi: 10.1002/pca.1031.
The feasibility of employing a non-ionic surfactant (Triton X-100) as an alternative and effective solvent for the microwave-assisted extraction of glycyrrhizic acid and liquiritin from liquorice root has been demonstrated. When compared with commonly used solvents, 5% Triton X-100 yielded higher extraction efficiency than aqueous solutions of ethanol or methanol. Under optimal conditions, i.e. 5% Triton X-100 (v/v) and microwave-assisted extraction for 3-5 min at 100 degrees C, the percentage extraction of active ingredients reached the highest value. The pre-concentration factor for the glycyrrhizic acid and liquiritin was about 13, and the cloud-point extraction recoveries for the two ingredients were 98.4 and 96.1%, respectively. The results showed that the coupling of microwave-assisted extraction and cloud-point extraction could be employed as a new and effective approach for the rapid extraction and pre-concentration of pharmacologically active ingredients from liquorice root without disturbing the subsequent chromatographic analysis.
已证明使用非离子表面活性剂（吐温X - 100）作为从甘草根中微波辅助提取甘草酸和甘草苷的替代有效溶剂的可行性。与常用溶剂相比，5%吐温X - 100的提取效率高于乙醇或甲醇的水溶液。在最佳条件下，即5%吐温X - 100（v/v），并在100℃下微波辅助提取3 - 5分钟，活性成分的提取率达到最高值。甘草酸和甘草苷的预浓缩因子约为13，两种成分的浊点萃取回收率分别为98.4%和96.1%。结果表明，微波辅助萃取与浊点萃取相结合可作为一种新的有效方法，用于从甘草根中快速提取和预浓缩药理活性成分，且不干扰后续的色谱分析。
