用于分光光度法测定药物制剂中十六烷基吡啶氯化物的选择性浊点萃取法。

Selective cloud point extraction for the spectrophotometric determination of cetylpyridinium chloride in pharmaceutical formulations.

作者信息

Zarei Ali Reza, Bagheri Sadeghi Hayedeh, Abedin Samira

机构信息

Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, 158751774-, Iran.

Department of Chemistry, Islamic Azad University, Tehran Central Branch, Tehran, Iran.

出版信息

Iran J Pharm Res. 2013 Fall;12(4):671-7.

In this work, we developed a simple and selective method for separation and spectrophotometric determination of trace amounts of cetylpyridinium chloride (CPC) in pharmaceutical products using cloud point extraction (CPE) technique. The method is based on cloud point extraction of the CPC in alkali conditions using of nonionic surfactant Triton X-114. Under optimal conditions, the calibration graph was linear in the range of 0.50-30 μg/ mL of CPC with r=0.9993 (n=10). Average recoveries for spiked samples were determined to be between 95-104%. The relative standard deviation (RSD) for 5.0 μg/mL of CPC was 1.86 % (n=10). Also, the use of micellar extraction for extracting CPC was enhanced the molar absorptivity (ε) from 1.83×10(3) L/mol.cm in aqueous solution to 1.539×10(4) L/mol.cm in surfactant-rich phase. The proposed method was applied for the determination of CPC in a commercial mouth washer product.

在本研究中，我们开发了一种简单且具有选择性的方法，利用浊点萃取（CPE）技术分离并分光光度法测定药品中痕量的十六烷基氯化吡啶（CPC）。该方法基于在碱性条件下使用非离子表面活性剂Triton X - 114对CPC进行浊点萃取。在最佳条件下，校准曲线在0.50 - 30 μg/mL的CPC范围内呈线性，r = 0.9993（n = 10）。加标样品的平均回收率测定为95% - 104%。5.0 μg/mL的CPC的相对标准偏差（RSD）为1.86%（n = 10）。此外，使用胶束萃取法萃取CPC可将摩尔吸光系数（ε）从水溶液中的1.83×10³ L/mol·cm提高到富含表面活性剂相中的1.539×10⁴ L/mol·cm。所提出的方法应用于测定一种市售漱口水产品中的CPC。