Xu Dongming, Cheng Kejian
Science and Technology Innovation of Small and Mid-sized Enterprise Fund Management Center, Ministry of Science and Technology, Beijing 10038, China.
Zhongguo Zhong Yao Za Zhi. 2011 Jan;36(1):45-7.
To develop a RP-HPLC method for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.
DIKMA Diamonsil(2)-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL min(-1). The detection wavelength was 237, 283 nm.
The linear responses ranged from 0.0199-0.1191 microg for liquiritin (r = 0.9997, n = 6), 0.1800-1.0800 microg for naringin (r = 0.9997, n = 5), 0.1455-0.8730 microg for hesperidin (r = 0.9998, n = 6), 0.0393-0.2355 microg for monoammonium glycyrrhizinate (r = 0.9997, n = 6), respectively. The average recoveries were 97.7% with RSD 1.5% for liquiritin, 97.7% with RSD 2.0% for naringin, 97.1% with RSD 2.0% for hesperidin and 98.5% with RSD 1.9% for glycyrrhizic acid, respectively.
The method is quick, simple and repeatable for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.
建立一种反相高效液相色谱法同时测定温胆汤提取物中甘草苷、柚皮苷、橙皮苷和甘草酸的含量。
采用迪马公司的 Diamonsil(2)-C18 柱（4.6 mm×250 mm，5 µm），柱温 25℃，以乙腈-0.1%磷酸为流动相进行梯度洗脱。流速设定为 1.0 mL·min⁻¹。检测波长为 237、283 nm。
甘草苷线性范围为 0.0199 - 0.1191 μg（r = 0.9997，n = 6），柚皮苷为 0.1800 - 1.0800 μg（r = 0.9997，n = 5），橙皮苷为 0.1455 - 0.8730 μg（r = 0.9998，n = 6），甘草酸单铵盐为 0.0393 - 0.2355 μg（r = 0.9997，n = 6）。甘草苷平均回收率为 97.7%，RSD 为 1.5%；柚皮苷平均回收率为 97.7%，RSD 为 2.0%；橙皮苷平均回收率为 97.1%，RSD 为 2.0%；甘草酸平均回收率为 98.5%，RSD 为 1.9%。
该方法快速、简便、重复性好，可用于同时测定温胆汤提取物中甘草苷、柚皮苷、橙皮苷和甘草酸的含量。
