[反相高效液相色谱法测定温胆方提取物中甘草苷、柚皮苷、橙皮苷及甘草酸的含量]
[Determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC].
作者信息
Xu Dongming, Cheng Kejian
机构信息
Science and Technology Innovation of Small and Mid-sized Enterprise Fund Management Center, Ministry of Science and Technology, Beijing 10038, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jan;36(1):45-7.
OBJECTIVE
To develop a RP-HPLC method for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.
METHOD
DIKMA Diamonsil(2)-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL min(-1). The detection wavelength was 237, 283 nm.
RESULT
The linear responses ranged from 0.0199-0.1191 microg for liquiritin (r = 0.9997, n = 6), 0.1800-1.0800 microg for naringin (r = 0.9997, n = 5), 0.1455-0.8730 microg for hesperidin (r = 0.9998, n = 6), 0.0393-0.2355 microg for monoammonium glycyrrhizinate (r = 0.9997, n = 6), respectively. The average recoveries were 97.7% with RSD 1.5% for liquiritin, 97.7% with RSD 2.0% for naringin, 97.1% with RSD 2.0% for hesperidin and 98.5% with RSD 1.9% for glycyrrhizic acid, respectively.
CONCLUSION
The method is quick, simple and repeatable for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.
目的
建立一种反相高效液相色谱法同时测定温胆汤提取物中甘草苷、柚皮苷、橙皮苷和甘草酸的含量。
方法
采用迪马公司的 Diamonsil(2)-C18 柱（4.6 mm×250 mm，5 µm），柱温 25℃，以乙腈-0.1%磷酸为流动相进行梯度洗脱。流速设定为 1.0 mL·min⁻¹。检测波长为 237、283 nm。
结果
甘草苷线性范围为 0.0199 - 0.1191 μg（r = 0.9997，n = 6），柚皮苷为 0.1800 - 1.0800 μg（r = 0.9997，n = 5），橙皮苷为 0.1455 - 0.8730 μg（r = 0.9998，n = 6），甘草酸单铵盐为 0.0393 - 0.2355 μg（r = 0.9997，n = 6）。甘草苷平均回收率为 97.7%，RSD 为 1.5%；柚皮苷平均回收率为 97.7%，RSD 为 2.0%；橙皮苷平均回收率为 97.1%，RSD 为 2.0%；甘草酸平均回收率为 98.5%，RSD 为 1.9%。
结论
该方法快速、简便、重复性好，可用于同时测定温胆汤提取物中甘草苷、柚皮苷、橙皮苷和甘草酸的含量。
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