Faucher Jean-Luc, Lacronique-Gazaille Charlotte, Frébet Elise, Trimoreau Franck, Donnard Magali, Bordessoule Dominique, Lacombe Francis, Feuillard Jean
Laboratoire d'Hématologie, Hôpital Dupuytren, Limoges, France.
Cytometry A. 2007 Nov;71(11):934-44. doi: 10.1002/cyto.a.20457.
Electronic white blood cell (WBC) differential by standard cytology (hematology analyzer and visual inspection of blood smears) is limited to five types and identification of abnormal cells is only qualitative, often problematic, poorly reproducible, and labour costing. We present our results on WBC differential by flow cytometry (FCM) with a 6 markers, 5 colors CD36-FITC/CD2-PE+CRTH2-PE/CD19-ECD/CD16-Cy5/CD45-Cy7 combination, on 379 subjects, with detection of 12 different circulating cell types, among them 11 were quantified. Detection of quantitative abnormalities of whole leucocytes, neutrophils, eosinophils, basophils, monocytes, or lymphocytes was comparable by FCM and by standard cytology in terms of sensitivity and specificity. FCM was better than standard cytology in detection and quantification of circulating blast cells or immature granulocytes, with a first lineage orientation in the former case. All cases of lymphocytosis, with lineage assignment, were detected by FCM. FCM identified a group of patients with excess of CD16pos monocytes as those having an inflammatory syndrome. WBC differential by FCM is at least as reliable as by standard cytology. FCM superiority consists in identification and systematic quantification of parameters that cannot be assessed by standard cytology such as lineage orientation of blast cells or lymphocytes, and expression of markers of interest such as CD16 on inflammatory monocytes.
通过标准细胞学方法（血液学分析仪和血涂片目视检查）进行的电子白细胞（WBC）分类仅限于五种类型，并且异常细胞的识别仅是定性的，通常存在问题，重复性差且成本高昂。我们展示了在379名受试者中使用6种标志物、5种颜色的CD36-FITC/CD2-PE+CRTH2-PE/CD19-ECD/CD16-Cy5/CD45-Cy7组合通过流式细胞术（FCM）进行白细胞分类的结果，检测到12种不同的循环细胞类型，其中11种进行了定量分析。在全白细胞、中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、单核细胞或淋巴细胞的定量异常检测方面，FCM和标准细胞学方法在敏感性和特异性方面相当。在检测和定量循环原始细胞或未成熟粒细胞方面，FCM优于标准细胞学方法，在前一种情况下具有初步的谱系定向。FCM检测到了所有伴有谱系分类的淋巴细胞增多病例。FCM将一组CD16阳性单核细胞增多的患者识别为患有炎症综合征的患者。通过FCM进行的白细胞分类至少与标准细胞学方法一样可靠。FCM的优势在于能够识别和系统定量标准细胞学方法无法评估的参数，如原始细胞或淋巴细胞的谱系定向，以及炎症单核细胞上如CD16等感兴趣标志物的表达。
