[糙叶败酱大孔吸附树脂提取物对荷瘤小鼠红细胞免疫功能的影响]
[Effects of Patrinia scabra bunge macroporous adsorptive resins extracts on erythrocyte immune function in tumor bearing mice].
作者信息
Wang Xue-xi, Zhao Jian-xiong, Cheng Wei-dong
机构信息
Institute of Integrated Traditional Chinese and Western Medicine, Lanzhou University, Lanzhou.
出版信息
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Aug;27(8):732-5.
OBJECTIVE
To study the erythrocyte immuno-regulatory effect of Patrinia scabra Bunge extracts extracted by macroporous adsorptive resins in tumor bearing mice.
METHODS
Patrinia scabra Bunge was extracted by macroporous adsorptive resins, and the amount of polysaccharides and saponins in the extract were determined. Mice bearing S180 tumor were treated with the extract and their survival prolongation rate, erythrocyte rosette formation rates of C3b receptor (ERR-CR), immune complex (ERR-IC) and tumor cell (ERR-TC), as well as the CD35 and CD44s were observed.
RESULTS
Polysaccharide content was 21.4%, saponin 41.8% in the extract. As compared with the model group, the survival rate was increased, the erythrocyte immune function was improved (showed increase of ERR-CR and ERR-TC, decrease of ERR-IC), and the amount of CD35 and CD44s in red blood cell membrane increased in mice after being treated with the extract (P < 0.05 or P < 0.01).
CONCLUSION
Extract of Patrinia scabra Bunge extracted by macroporous adsorptive resins can regulate the erythrocyte immune function to a certain extent.
目的
研究大孔吸附树脂提取的糙叶败酱提取物对荷瘤小鼠红细胞免疫调节的作用。
方法
采用大孔吸附树脂提取糙叶败酱，测定提取物中多糖和皂苷的含量。用提取物处理荷S180肿瘤小鼠，观察其生存延长率、C3b受体红细胞花环形成率（ERR-CR）、免疫复合物红细胞花环形成率（ERR-IC）和肿瘤细胞红细胞花环形成率（ERR-TC），以及CD35和CD44s的表达情况。
结果
提取物中多糖含量为21.4%，皂苷含量为41.8%。与模型组相比，提取物处理后的小鼠生存率提高，红细胞免疫功能改善（ERR-CR和ERR-TC升高，ERR-IC降低），红细胞膜上CD35和CD44s的表达量增加（P<0.05或P<0.01）。
结论
大孔吸附树脂提取的糙叶败酱提取物能在一定程度上调节红细胞免疫功能。
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