College of Animal Science, Northwest Agriculture and Forestry University, Yangling, Shaanxi Province, China.
Pharm Biol. 2010 Sep;48(9):1012-7. doi: 10.3109/13880200903437852.
The research investigated the effect of Patrinia heterophylla Bunge (Valerianaceae) polysaccharides (PHB-P1) on U14-bearing mice. The tumor weight of mice treated with PHB-P1 (30, 60 mg/kg body weight) was significantly lower than that of the control group, a decrease of serum lactate dehydrogenase (LDH) activity was observed, and the serum alkaline phosphatase (AKP) level was increased slightly. The number of apoptotic tumor cells was significantly increased in the mice by treatment of PHB-P1 (30, 60 mg/kgbw). Cell cycle analysis showed the accumulation of tumor cells in the G2/M phase and a relative decrease of the S phase. By the immunohistochemical analysis, PHB-P1 (30, 60 mg/kgbw) might up-regulate the expression of p53 and Bax, and significantly inhibited the expression of Bcl-2 in tumor tissues. In conclusion, PHB-P1 could inhibit tumor growth and induce tumor cell apoptosis.
该研究调查了白花败酱草多糖（PHB-P1）对 U14 荷瘤小鼠的影响。用 PHB-P1（30、60mg/kg 体重）处理的小鼠的肿瘤重量明显低于对照组，观察到血清乳酸脱氢酶（LDH）活性降低，血清碱性磷酸酶（AKP）水平略有升高。PHB-P1（30、60mg/kgbw）处理可显著增加凋亡肿瘤细胞的数量。细胞周期分析显示肿瘤细胞在 G2/M 期积聚，S 期相对减少。通过免疫组化分析，PHB-P1（30、60mg/kgbw）可能上调 p53 和 Bax 的表达，并显著抑制肿瘤组织中 Bcl-2 的表达。综上所述，PHB-P1 可抑制肿瘤生长并诱导肿瘤细胞凋亡。
