School of Pharmaceutical Science, Gansu University of Traditional Chinese Medicine, Lanzhou, 730000, Gansu, China.
Gansu Provincial Academy of Medical Sciences, Lanzhou, 730000, Gansu, China.
BMC Complement Med Ther. 2022 Jan 4;22(1):7. doi: 10.1186/s12906-021-03489-7.
Patrinia scabra Bunge is a well-known herbal medicine for its favorable treatment on inflammatory diseases owing to its effective ingredients, in which iridoid glycoside plays an extremely significant role. This article aimed to improve the content of total iridoid glycosides in crude extract through a series optimization of extraction procedure. Moreover, considering that both pain and inflammation are two correlated responses triggered in response to injury, irritants or pathogen, the article investigated the anti-inflammatory and analgesic activities of P. scabra to screen out the active fraction.
P. scabra was extracted by ultrasonic-microwave synergistic extraction (UMSE) to obtain total iridoid glycosides (PSI), during which a series of conditions were investigated based on single-factor experiments. The extraction process was further optimized by a reliable statistical method of response surface methodology (RSM). The elution fractions of P. scabra extract were prepared by macroporous resin column chromatography. Through the various animal experiment including acetic acid-induced writhing test, formalin induced licking and flinching, carrageenan-induced mice paw oedema test and xylene-induced ear edema in mice, the active fractions with favorable analgesic and anti-inflammatory effect were reasonably screen out.
The content of PSI could reach up to 81.42 ± 0.31 mg/g under the optimum conditions as follows: ethanol concentration of 52%, material-to-liquid ratio of 1:18 g/mL, microwave power at 610 W and extraction time of 45 min. After gradient elution by the macroporous resin, the content of PSI increased significantly. Compared with other concentrations of elution liquid, the content of PSI in 30 and 50% ethanol eluate was increased to reach 497.65 and 506.90 mg/g, respectively. Owing to the pharmacology experiment, it was reasonably revealed that 30 and 50% ethanol elution fractions of P. scabra could relieve pain centrally and peripherally, exhibiting good analgesic and anti-inflammatory activities.
Patrinia scabra possessed rich iridoids and exhibited significant analgesic and anti-inflammatory activities.
苦地丁是一种著名的草药，由于其有效成分，对炎症性疾病有很好的治疗作用，其中环烯醚萜苷类化合物起着极其重要的作用。本文旨在通过一系列优化提取工艺，提高粗提取物中环烯醚萜总苷的含量。此外，由于疼痛和炎症都是对损伤、刺激物或病原体的两种相关反应，本文研究了苦地丁的抗炎和镇痛活性，以筛选出有效部位。
采用超声-微波协同提取（UMSE）法提取总环烯醚萜苷（PSI），在单因素实验的基础上，考察了一系列条件。采用可靠的响应面法（RSM）对提取工艺进行了进一步优化。采用大孔树脂柱色谱法制备苦地丁提取物的洗脱部位。通过醋酸诱导扭体试验、甲醛诱导舔咬和退缩试验、角叉菜胶诱导小鼠足肿胀试验和二甲苯诱导小鼠耳肿胀试验等各种动物实验，合理筛选出具有良好镇痛和抗炎作用的活性部位。
在最佳条件下，PSI 的含量可达到 81.42±0.31mg/g，条件为：乙醇浓度 52%、料液比 1:18g/ml、微波功率 610W、提取时间 45min。经大孔树脂梯度洗脱后，PSI 含量明显增加。与其他浓度洗脱液相比，30%和 50%乙醇洗脱液中 PSI 的含量分别增加到 497.65 和 506.90mg/g。由于药理实验合理揭示了苦地丁 30%和 50%乙醇洗脱部位可通过中枢和外周途径缓解疼痛，具有良好的镇痛和抗炎活性。
苦地丁含有丰富的环烯醚萜类化合物，具有显著的镇痛和抗炎活性。
