Kilík R, Bober J, Gál P, Vidinský B, Mokrý M, Longauer F, Sabo J
1. Chirurgická klinika, Lekárska fakulta, Univerzity Pavla Jozefa Safárika v Kosiciach, Slovenská republika,
Rozhl Chir. 2007 Jul;86(7):384-7.
The optimal parameters of low level laser therapy (LLLT) are still under debate. It has been documented that a dose or 5 J/cm2 would be capable to accelerate the wound healing process in patients. However, the optimal delivering form, i.e. power intensity, is unknown. Therefore, the aim of our study was to compare different power densities of LLLT.
Sixteen male Sprague-Dawley rats were included in this experiment and randomized into two groups, normal healthy group and streptozotocine induced diabetic group. In general anesthesia four full thickness skin incisions were performed under standard aseptic conditions on the back of each rat and immediately closed using intradermal running suture. Three wounds were stimulated with diode laser (wavelength: 635 nm; daily dose 5 J/cm2; power densities: 1 mW/cm2, 5 mW/cm2 and 15 mW/cm2) each with different power density while the fourth wound served as control. Six days after surgery animals were sacrificed and samples removed for histological evaluation.
Our study demonstrated that LLLT positively influences wound healing. The most significant changes were observed in wounds stimulated at the highest power density 15 mW/cm2. Since using the highest power density the shortest time is needed to achieve the optimal daily dose of 5 J/cm2, it can be suggested that 15 mW/cm2 might be optimal parameter for such a therapy in patients.
低强度激光治疗（LLLT）的最佳参数仍存在争议。已有文献记载，5 J/cm² 的剂量能够加速患者伤口愈合过程。然而，最佳的递送形式，即功率强度，尚不清楚。因此，我们研究的目的是比较低强度激光治疗的不同功率密度。
本实验纳入了16只雄性Sprague-Dawley大鼠，并随机分为两组，正常健康组和链脲佐菌素诱导的糖尿病组。在全身麻醉下，在每只大鼠背部的标准无菌条件下进行四个全层皮肤切口，并用皮内连续缝合立即闭合。三个伤口分别用不同功率密度的二极管激光（波长：635 nm；每日剂量5 J/cm²；功率密度：1 mW/cm²、5 mW/cm² 和15 mW/cm²）刺激，而第四个伤口作为对照。术后六天处死动物，取出样本进行组织学评估。
我们的研究表明，低强度激光治疗对伤口愈合有积极影响。在以最高功率密度15 mW/cm² 刺激的伤口中观察到最显著的变化。由于使用最高功率密度时达到5 J/cm² 的最佳每日剂量所需时间最短，因此可以认为15 mW/cm² 可能是该治疗方法对患者的最佳参数。
