Wu Xingjia, Alberico Stephanie, Saidu Edward, Rahman Khan Sazzadur, Zheng Shijun, Romero Rebecca, Sik Chae Hyun, Li Sheng, Mochizuki Amane, Anders Juanita
Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Wound Repair Regen. 2015 Jan-Feb;23(1):104-14. doi: 10.1111/wrr.12258. Epub 2015 Mar 31.
A major complication for diabetic patients is chronic wounds due to impaired wound healing. It is well documented that visible red wavelengths can accelerate wound healing in diabetic animal models and patients. In vitro and in vivo diabetic models were used to investigate the effects of organic light emitting diode (OLED) irradiation on cellular function and cutaneous wound healing. Human dermal fibroblasts were cultured in hyperglycemic medium (glucose concentration 180 mM) and irradiated with an OLED (623 nm wavelength peak, range from 560 to 770 nm, power density 7 or 10 mW/cm2 at 0.2, 1, or 5 J/cm2). The OLED significantly increased total adenosine triphosphate concentration, metabolic activity, and cell proliferation compared with untreated controls in most parameters tested. For the in vivo experiment, OLED and laser (635 ± 5 nm wavelength) treatments (10 mW/cm2 , 5 J/cm2 daily for a total of seven consecutive days) for cutaneous wound healing were compared using a genetic, diabetic rat model. Both treatments had significantly higher percentage of wound closure on day 6 postinjury and higher total histological scores on day 13 postinjury compared with control. No statistical difference was found between the two treatments. OLED irradiation significantly increased fibroblast growth factor-2 expression at 36-hour postinjury and enhanced macrophage activation during initial stages of wound healing. In conclusion, the OLED and laser had comparative effects on enhancing diabetic wound healing.
糖尿病患者的一个主要并发症是由于伤口愈合受损导致的慢性伤口。有充分的文献记载，可见红光波长可加速糖尿病动物模型和患者的伤口愈合。使用体外和体内糖尿病模型来研究有机发光二极管（OLED）照射对细胞功能和皮肤伤口愈合的影响。将人真皮成纤维细胞在高糖培养基（葡萄糖浓度180 mM）中培养，并用OLED（波长峰值623 nm，范围560至770 nm，功率密度7或10 mW/cm²，剂量0.2、1或5 J/cm²）照射。在大多数测试参数中，与未处理的对照组相比，OLED显著提高了总三磷酸腺苷浓度、代谢活性和细胞增殖。对于体内实验，使用遗传性糖尿病大鼠模型比较了OLED和激光（波长635±5 nm）治疗（10 mW/cm²，每天5 J/cm²，连续7天）对皮肤伤口愈合的效果。与对照组相比，两种治疗在受伤后第6天的伤口闭合百分比均显著更高，在受伤后第13天的组织学总评分也更高。两种治疗之间未发现统计学差异。OLED照射在受伤后36小时显著增加了成纤维细胞生长因子-２的表达，并在伤口愈合的初始阶段增强了巨噬细胞的激活。总之，OLED和激光在促进糖尿病伤口愈合方面具有相似的效果。
