Wen Tao, Liang Li, Zeng Yang, Yu Xiao
Agronomy College, Sichuan Agricultural University, Ya' an 625014, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jul;32(13):1277-80.
To study the effect of light intensity (LI) on the callus induction and resveratrol content of Polygonum cuspidatum.
Taking leaves and stem segment of P. cuspidatum as explants, the callus was inducted and cultured under different LI. The growth status of callus was observed and analyzed. The content of resveratrol was determined by HPLC.
The formation and growth of the stem segment callus were optimal under low light treatment (1340-1560 lx). The resveratrol content was 18.350 microg x g(-1) in fresh stem segment and 140.074 microg x g(-1) in dry stem segment, which was two times higher than that of wild P. cuspidatum.
Introduction and status of P. cuspidatum callus were obvious diverse under different LI, the resveratrol content of P. cuspidatum was enhanced under appropriate LI.
研究光照强度对虎杖愈伤组织诱导及白藜芦醇含量的影响。
以虎杖叶片和茎段为外植体，在不同光照强度下诱导并培养愈伤组织。观察并分析愈伤组织的生长状况。采用高效液相色谱法测定白藜芦醇含量。
在低光照处理（1340 - 1560勒克斯）下，茎段愈伤组织的形成与生长最佳。新鲜茎段中白藜芦醇含量为18.350微克×克⁻¹，干燥茎段中为140.074微克×克⁻¹，比野生虎杖高出两倍。
不同光照强度下虎杖愈伤组织的诱导情况及状态明显不同，适宜光照强度可提高虎杖白藜芦醇含量。
