Bu Xiao-Ying, Dong Ai-Wen, Guan Qiong-Yu, Wu Feng
Forest Products Chemical Engineering Key Laboratory of Hunan Province, Jishou University, Zhangjiajie 427000, China.
Zhong Yao Cai. 2012 Dec;35(12):1909-13.
To establish fluorescence microscopy combined with HPLC method for rapid detection the distribution and content of resveratrol tissues in different growth stages of Polygonum cuspidatum.
Used sequential experiment to design conditions of frozen and observe of the section by fluorescence microscopy; Resveratrol was extracted by ultrasonic-assisted extraction and its content was detected by HPLC.
The results showed that frozen condition for concentration of gum Arabic was from 20% (dipping time was 5 - 6 h) to 40% (2 - 5 min), the freezer temperature was -5 degrees C, and the thickness was 15 microm. Resveratrol in polygonum cuspidatum was mainly accumulated in the organs, tissues and cells of fiber and cellulose, its content in rhizomes declined as the following sequence: spinal cord > xylem > phloem > periderm; Its content declined in organ as the following sequence: buds > rhizomes > ground stem > leaves; The content of resveratrol in root increased with age.
The results of fluorescence microscopic observation is in accordance with the HPLC results, indicating that the method is simple, fast and reliable, and provides a fast and reliable detection method for the determination of optimum harvesting period of Polygonum cuspidatum and acquisition of quality.
建立荧光显微镜结合高效液相色谱法快速检测虎杖不同生长阶段组织中白藜芦醇的分布及含量。
采用序贯试验设计冰冻条件并通过荧光显微镜观察切片；采用超声辅助提取法提取白藜芦醇，并用高效液相色谱法检测其含量。
结果表明，阿拉伯胶浓度的冰冻条件为20%（浸渍时间为5 - 6小时）至40%（2 - 5分钟），冷冻温度为-5℃，厚度为15微米。虎杖中的白藜芦醇主要积累在纤维和纤维素的器官、组织和细胞中，其在根茎中的含量按以下顺序下降：髓>木质部>韧皮部>周皮；其在器官中的含量按以下顺序下降：芽>根茎>地上茎>叶；根中白藜芦醇的含量随年龄增长而增加。
荧光显微镜观察结果与高效液相色谱结果一致，表明该方法简便、快速、可靠，为虎杖最佳采收期的确定和品质获取提供了一种快速可靠的检测方法。
