Yang Sihua, Xing Da, Zhou Quan, Xiang Liangzhong, Lao Yeqi
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China.
Med Phys. 2007 Aug;34(8):3294-301. doi: 10.1118/1.2757088.
Photoacoustic imaging (PAI) is a noninvasive, nonionizing modality based on the differences in light absorption of various biological tissues. PAI utilizes the endogenous contrast characteristics of traditional optical imaging, while benefiting from high spatial resolution of the ultrasound imaging. A PAI system was developed to reconstruct the two-dimensional cross section image and to visualize the cerebrovascular activities of mouse in vivo. The spatial resolution of the PAI system was determined to be 0.110 mm by a two-point-source phantom with the Rayleigh criterion. The potential applications of the system were clearly demonstrated by successfully mapping a traumatic lesion in the mouse brain cerebral cortex, by its ability to monitor physiological changes in the brain due to carotid ligation and drug stimulation, and two-dimensional sliced images of a traumatic mouse brain at different depths were also provided. Our experimental results indicate that PAI has the potential for studying of traumatic brain injury and physiological functions of the brain.
光声成像（PAI）是一种基于各种生物组织光吸收差异的非侵入性、非电离成像方式。PAI利用传统光学成像的内源性对比特性，同时受益于超声成像的高空间分辨率。开发了一种PAI系统来重建二维横截面图像并可视化小鼠体内的脑血管活动。通过使用瑞利准则的双点源体模，确定PAI系统的空间分辨率为0.110毫米。该系统的潜在应用通过成功绘制小鼠大脑皮层的创伤性病变、监测颈动脉结扎和药物刺激引起的大脑生理变化的能力得到了明确证明，并且还提供了不同深度的创伤小鼠大脑的二维切片图像。我们的实验结果表明，PAI在研究创伤性脑损伤和大脑生理功能方面具有潜力。
