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蜂胶生物活性成分的不同提取方法:一项初步研究。

Different extraction methods of biologically active components from propolis: a preliminary study.

作者信息

Trusheva Boryana, Trunkova Dorina, Bankova Vassya

机构信息

Institute of Organic Chemistry with the Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.

出版信息

Chem Cent J. 2007 Jun 7;1:13. doi: 10.1186/1752-153X-1-13.

DOI:10.1186/1752-153X-1-13
PMID:17880743
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1994058/
Abstract

BACKGROUND

Propolis is widely used in apitherapy, preparations, and food and beverage additives. Various extraction techniques were applied in the extraction of the biologically active constituents of poplar type propolis in order to compare their efficiency. The methods employed were: traditional maceration extraction, ultrasound extraction (UE), and microwave assisted extraction (MAE).

RESULTS

The total amounts of extracted phenolics and flavonoids were determined, and the effectiveness of the methods compared. MAE was very rapid but led to the extraction of a large amount of non-phenolic and non-flavonoid material. UE gave the highest percentage of extracted phenolics.

CONCLUSION

Compared to the maceration extraction, MAE and UE methods provided high extraction yield, requiring short timeframes and less labour. UE was shown to be the most efficient method based on yield, extraction time and selectivity.

摘要

背景

蜂胶广泛应用于蜂疗、制剂以及食品和饮料添加剂中。为比较不同提取技术的效率,采用了多种方法提取杨树型蜂胶的生物活性成分。所采用的方法有:传统浸渍提取法、超声提取法(UE)和微波辅助提取法(MAE)。

结果

测定了提取的酚类和黄酮类化合物的总量,并比较了这些方法的有效性。微波辅助提取法速度非常快,但会提取出大量非酚类和非黄酮类物质。超声提取法提取的酚类化合物百分比最高。

结论

与浸渍提取法相比,微波辅助提取法和超声提取法提取效率高,所需时间短且劳动力少。基于产量、提取时间和选择性,超声提取法被证明是最有效的方法。

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本文引用的文献

1
Preparation of Water and Ethanolic Extracts of Propolis and Evaluation of the Preparations.蜂胶水提取物和乙醇提取物的制备及其制剂评价
Biosci Biotechnol Biochem. 1998;62(11):2230-2. doi: 10.1271/bbb.62.2230.
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Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition.土耳其蜂胶的抗菌活性及其定性和定量化学成分。
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3
Validated methods for the quantification of biologically active constituents of poplar-type propolis.杨树型蜂胶生物活性成分定量的验证方法。
Phytochem Anal. 2004 Jul-Aug;15(4):235-40. doi: 10.1002/pca.777.
4
Recent extraction techniques for natural products: microwave-assisted extraction and pressurised solvent extraction.天然产物的最新提取技术:微波辅助提取和加压溶剂提取。
Phytochem Anal. 2002 Mar-Apr;13(2):105-13. doi: 10.1002/pca.631.
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A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants.用于药用植物提取与分析的现代样品制备技术综述。
Anal Bioanal Chem. 2002 May;373(1-2):23-30. doi: 10.1007/s00216-002-1265-3. Epub 2002 Apr 3.