Popova M, Silici S, Kaftanoglu O, Bankova V
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Phytomedicine. 2005 Mar;12(3):221-8. doi: 10.1016/j.phymed.2003.09.007.
The antibacterial activity of propolis from different regions of Turkey was studied, accompanied by TLC and GC-MS analyses of its chemical composition and spectrophotometric quantification of the most important active principles. All six samples were active against the bacterial test strains used; however, samples 1 (Yozgat), 2 (Izmir) and 3 (Kayseri) were more active than samples 4 (Adana), 5 (Erzurum) and 6 (Artvin). By TLC comparison all samples were found to contain poplar taxonomic markers but in samples 4 (Adana), 5 (Erzurum) and 6 (Artvin), different substances were observed, which were not present in P. nigra L. bud exudate. The typical poplar samples 1 (Yozgat), 2 (Izmir) and 3 (Kayseri) displayed very similar phenolic and flavonoid content. Samples 4 (Adana), 5 (Erzurum) and 6 (Artvin) were characterized by low phenolic and very low flavonoid concentrations. Qualitative analysis by GC-MS revealed that sample 4 (Adana) contained diterpenic acids and high percent of cinnamyl cinnamate, sample 5 (Erzurum)-significant amounts of hydroxy fatty acids and triterpenic alcohoLs, and sample 6 (Artvin)-phenolic glycerides, characteristic for the bud exudate of Populus euphratica Oliv. The results confirm the importance of phenolics for propolis antibacterial activity, and the significance of P. nigra L. as a propolis source, which provides the hive with the best defense against microorganisms.
研究了来自土耳其不同地区的蜂胶的抗菌活性，并对其化学成分进行了薄层色谱（TLC）和气相色谱 - 质谱联用（GC - MS）分析，同时对最重要的活性成分进行了分光光度法定量。所有六个样品对所使用的细菌测试菌株均有活性；然而，样品1（约兹加特）、2（伊兹密尔）和3（开塞利）比样品4（阿达纳）、5（埃尔祖鲁姆）和6（阿尔特温）更具活性。通过TLC比较发现，所有样品均含有杨树分类标记，但在样品4（阿达纳）、5（埃尔祖鲁姆）和6（阿尔特温）中观察到了黑杨（P. nigra L.）芽渗出物中不存在的不同物质。典型的杨树样品1（约兹加特）、2（伊兹密尔）和3（开塞利）显示出非常相似的酚类和黄酮类含量。样品4（阿达纳）、5（埃尔祖鲁姆）和6（阿尔特温）的特征是酚类浓度低且黄酮类浓度极低。GC - MS定性分析表明，样品4（阿达纳）含有二萜酸和高比例的肉桂酸肉桂酯，样品5（埃尔祖鲁姆）含有大量的羟基脂肪酸和三萜醇，样品6（阿尔特温）含有胡杨（Populus euphratica Oliv.）芽渗出物特有的酚类甘油酯。结果证实了酚类物质对蜂胶抗菌活性的重要性，以及黑杨作为蜂胶来源的重要性，它为蜂巢提供了抵御微生物的最佳防御。
