Rosenberg N, Baltimore D
J Exp Med. 1976 Jun 1;143(6):1453-63. doi: 10.1084/jem.143.6.1453.
A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction.
已利用半固体琼脂糖培养系统开发出一种定量阿贝尔森鼠白血病病毒(A-MuLV)淋巴细胞转化试验。在这些条件下,淋巴细胞转化显示出与所用A-MuLV剂量呈线性变化。使用琼脂糖试验可估计来自不同品系小鼠的骨髓细胞对A-MuLV诱导转化的敏感性。可以鉴定出对A-MuLV具有高、中、低敏感性的骨髓细胞品系。该试验已用于研究来自胎儿和成年小鼠淋巴器官的细胞对A-MuLV的敏感性。来自胎儿肝脏、成年骨髓和成年脾脏的细胞悬液对A-MuLV敏感,而胸腺细胞对A-MuLV诱导的转化具有抗性。在用A-MuLV感染之前对骨髓细胞进行牛血清白蛋白梯度分级分离表明,大多数对A-MuLV敏感的细胞在一条宽带中回收,该宽带部分与大多数有核细胞重叠。琼脂糖试验系统允许在单细胞-单病毒颗粒相互作用水平上研究A-MuLV与淋巴细胞的相互作用。