阿贝尔森白血病病毒在体外引发小鼠淋巴细胞的致癌转化

Sklar M D, White B J, Rowe W P

Proc Natl Acad Sci U S A. 1974 Oct;71(10):4077-81. doi: 10.1073/pnas.71.10.4077.

While C-type RNA viruses are known to induce leukemias and lymphomas, oncogenic transformation of lymphoid cells by them in vitro has not been reported. In this study, splenocytes from female BALB/c mice were infected in vitro with Abelson virus, a C-type RNA virus that induces nonthymic lymphomas and plasmacytomas in mice. The cells were transplanted into recipients of different karyotype, either male BALB/c mice or hybrid BALB/c x AL/N (CALF1) mice, which bear the Rb(5.19)1Wh translocation. Transplants of eight of the resulting tumors (one plasmacytoma and seven lymphomas) contained cells of donor BALB/c karyotype, indicating that transformation of splenocytes occurred in vitro.

虽然已知C型RNA病毒可诱发白血病和淋巴瘤，但尚未见其在体外使淋巴样细胞发生致癌转化的报道。在本研究中，用Abelson病毒（一种在小鼠中诱发非胸腺淋巴瘤和浆细胞瘤的C型RNA病毒）体外感染雌性BALB/c小鼠的脾细胞。将这些细胞移植到不同核型的受体小鼠中，即雄性BALB/c小鼠或带有Rb(5.19)1Wh易位的杂种BALB/c×AL/N（CALF1）小鼠。所产生的8个肿瘤（1个浆细胞瘤和7个淋巴瘤）移植体含有供体BALB/c核型的细胞，表明脾细胞的转化发生在体外。