用粗制分枝杆菌蛋白70进行牛结核病的酶联免疫吸附测定

Enzyme-linked immunosorbent assay of bovine tuberculosis by crude mycobacterial protein 70.

作者信息

Cho Yun Sang, Jung Suk Chan, Kim Jong Man, Yoo Han Sang

机构信息

Bacteriology and Parasitology Division, Animal Disease Research Department, National Veterinary Research and Quarantine Service, Mannan-gu, Anyang, Gyeonggi-do, Republic of Korea.

出版信息

J Immunoassay Immunochem. 2007;28(4):409-18. doi: 10.1080/15321810701603781.

DOI:10.1080/15321810701603781

PMID:17885893

Abstract

MPB70 (mycobacterial protein of BCG 70) as T-cell stimulator has been tried with an intradermal skin test (IST) and enzyme-linked immunosorbent assay (ELISA) of bovine tuberculosis (BTB). In this study, crude mycobacterial protein 70 (CMP70) was prepared by anion exchange chromatography from the culture supernatant of Mycobacterium bovis AN5 and CMP70 ELISA was compared with purified protein derivative (PPD) ELISA. PPD and CMP70 ELISA have shown a positive reaction to the sera of M. bovis infected cattle and IST positive reactors. One of three IST negative cattle showed the nonspecific reaction in PPD ELISA, whereas all of the IST negative cattle (n=3) were did not show the nonspecific reaction in CMP70 ELISA. When each ELISA was applied to sixty-two IST positive cattle, ELISA positive reactors were eighty four per cent to CMP70 antigen and fifty-two per cent to PPD. CMP70 has been shown to be more specific and sensitive than PPD in ELISA.

摘要

作为T细胞刺激剂的MPB70（卡介苗70kDa分枝杆菌蛋白）已通过皮内皮肤试验（IST）和牛结核病（BTB）的酶联免疫吸附测定（ELISA）进行了试验。在本研究中，通过阴离子交换色谱法从牛分枝杆菌AN5的培养上清液中制备了粗分枝杆菌蛋白70（CMP70），并将CMP70 ELISA与纯化蛋白衍生物（PPD）ELISA进行了比较。PPD ELISA和CMP70 ELISA对牛分枝杆菌感染牛的血清和IST阳性反应动物均呈阳性反应。三只IST阴性牛中的一只在PPD ELISA中出现非特异性反应，而所有IST阴性牛（n = 3）在CMP70 ELISA中均未出现非特异性反应。当将每种ELISA应用于62只IST阳性牛时，ELISA对CMP70抗原的阳性反应动物为84%，对PPD的为52%。在ELISA中，CMP70已被证明比PPD更具特异性和敏感性。