Department of Microbiology and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 120-752 Seoul, Republic of Korea.
Res Vet Sci. 2010 Jun;88(3):390-3. doi: 10.1016/j.rvsc.2009.12.002. Epub 2010 Jan 8.
An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of Mycobacterium bovis was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (R(2)=0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8% and 81.8%, respectively, and the specificities were 97.7% and 100%, respectively. In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5% (42/931) and the tuberculin-positive rate was 2.8% (26/931). Individual milk samples (n=253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5% (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1% (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows.
一种使用大罐奶样的酶联免疫吸附试验（ELISA）被评估为牛结核病（TB）的筛查试验，牛结核病是一种传染性的慢性牛病。使用 MPB70（牛分枝杆菌的主要抗原）对 33 头结核菌素阳性和 43 头结核菌素阴性牛的配对奶样和血清样进行了 ELISA 检测。在奶样中检测到抗-MPB70 抗体，奶样和血清样的血清学反应之间存在显著相关性（R²=0.83）。使用结核菌素皮试作为参考试验，ELISA 检测奶样和血清样的敏感性分别为 87.8%和 81.8%，特异性分别为 97.7%和 100%。在韩国京畿道华城市的 931 个奶牛场的大罐奶样筛查试验中，抗-MPB70 抗体的阳性率为 4.5%（42/931），结核菌素阳性率为 2.8%（26/931）。从随机选择的 8 个有问题的和 3 个阴性牛场（分别为使用大罐奶样的 MPB70 ELISA 进行的筛查试验阳性和阴性）中采集了 253 份个体奶样，并使用 MPB70 奶 ELISA 进行了检测。在有问题的牛场中，20/190（10.5%）的奶 ELISA 检测到抗-MPB70 抗体阳性，2/190（2.1%）的结核菌素皮试阳性。在有问题的牛场中，有不止一头奶牛的奶 ELISA 检测呈阳性，所有结核菌素阳性奶牛的奶 ELISA 检测均呈阳性。此外，在阴性牛场中，无论是奶 ELISA 还是结核菌素皮试均未检测到阳性奶牛。这些结果表明，使用大罐奶样的 ELISA 可能是一种用于奶牛牛结核病的潜在有效筛查试验。
