Ku Bok Kyung, Jeon Bo-Young, Kim Jae Myung, Jang Young-Boo, Lee Hyeyoung, Choi Jae Young, Jung Suk Chan, Nam Hyang-Mi, Park Hun, Cho Sang-Nae
Bacterial Disease, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju 26493, Korea.
J Vet Sci. 2018 Jan 31;19(1):45-50. doi: 10.4142/jvs.2018.19.1.45.
Bovine tuberculosis is a chronic contagious disease responsible for major agricultural economic losses. Abattoir monitoring and trace-back systems are an appropriate method to control bovine tuberculosis, particularly in beef cattle. In the present study, a trace-back system was applied to bovine tuberculosis cases in Korean native Hanwoo beef cattle. Bovine tuberculosis was detected in three index beef cattle during abattoir monitoring in Jeonbuk Province, Korea, and the original herds were traced back from each index cow. All cattle in each original herd were subjected to tuberculin skin test. The positive rates in the tuberculin skin test were 64.6% (62 of 96), 4.8% (2 of 42), and 8.1% (3 of 37) at farms A, B, and C, respectively. On post-mortem examination of 56 tuberculin-positive cattle, 62% had granulomatous lesions, and was cultured from 40 (71.4%) of the cattle. Molecular typing by spoligotyping and the mycobacterial interspersed repetitive unit-variable-number tandem repeat assay revealed the genotype of the strains from the index cattle were same as the genotype in each original herd. The results suggest that tracing back from index cattle to the original herd is an effective method to control bovine tuberculosis in beef cattle.
牛结核病是一种慢性传染病，会造成重大农业经济损失。屠宰场监测和追溯系统是控制牛结核病的一种合适方法，尤其是在肉牛方面。在本研究中，一个追溯系统被应用于韩国本土韩牛肉牛的牛结核病例。在韩国全罗北道的屠宰场监测期间，在三头索引肉牛中检测到牛结核病，并从每头索引牛追溯其原牛群。对每个原牛群中的所有牛进行结核菌素皮肤试验。在A、B和C农场，结核菌素皮肤试验的阳性率分别为64.6%（96头中的62头）、4.8%（42头中的2头）和8.1%（37头中的3头）。在对56头结核菌素阳性牛进行尸检时，62%有肉芽肿病变，并且从40头（71.4%）牛中培养出了[具体细菌名称未给出]。通过间隔寡核苷酸分型（spoligotyping）和分枝杆菌插入重复单位可变数目串联重复序列分析（mycobacterial interspersed repetitive unit-variable-number tandem repeat assay）进行分子分型显示，索引牛的[细菌名称未给出]菌株基因型与每个原牛群中的[细菌名称未给出]基因型相同。结果表明，从索引牛追溯到原牛群是控制肉牛牛结核病的有效方法。
