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一种用于筛选耐万古霉素屎肠球菌和粪肠球菌的新型显色琼脂培养基——chromID VRE。

A new chromogenic agar medium, chromID VRE, to screen for vancomycin-resistant Enterococcus faecium and Enterococcus faecalis.

作者信息

Ledeboer Nathan A, Tibbetts Robert J, Dunne William M

机构信息

Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, Campus Box 8118, St. Louis, MO 63110, USA.

出版信息

Diagn Microbiol Infect Dis. 2007 Dec;59(4):477-9. doi: 10.1016/j.diagmicrobio.2007.06.018. Epub 2007 Sep 21.

Abstract

We compared the performance of a chromogenic agar medium chromID VRE (bioMérieux, Marcy-l'Etoile, France) designed to recover and identify vancomycin-resistant enterococci (VRE) from clinical specimens with bile esculin azide vancomycin (BEAV) agar. For this study, 120 stool specimens were plated on chromID VRE and BEAV and examined after 24 and 48 h. At 24 h, the sensitivity and specificity were as follows: BEAV, 90.2% and 73%, respectively; chromID VRE, 86.3% and 100.0%, respectively. Furthermore, we determined that the sensitivity and specificity of chromID VRE for Enterococcus faecium were 85.4% and 100%, respectively, and for Enterococcus faecalis, 90% and 100%, respectively. We conclude that chromID VRE provides an equivalent sensitivity for the recovery of VRE from stool specimens, with improved specificity, and the added advantage of providing differentiation between vancomycin-resistant E. faecium and E. faecalis.

摘要

我们比较了一种用于从临床标本中分离和鉴定耐万古霉素肠球菌(VRE)的显色琼脂培养基chromID VRE(法国生物梅里埃公司,马西 - 莱图瓦勒)与胆汁七叶苷叠氮万古霉素(BEAV)琼脂的性能。在本研究中,将120份粪便标本接种于chromID VRE和BEAV上,并在24小时和48小时后进行检查。在24小时时,敏感性和特异性如下:BEAV分别为90.2%和73%;chromID VRE分别为86.3%和100.0%。此外,我们确定chromID VRE对粪肠球菌的敏感性和特异性分别为85.4%和100%,对屎肠球菌分别为90%和100%。我们得出结论,chromID VRE从粪便标本中分离VRE的敏感性相当,特异性有所提高,并且具有区分耐万古霉素粪肠球菌和屎肠球菌的额外优势。

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