Asir K, Wilkinson K, Perry J D, Reed R H, Gould F K
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, UK.
Lett Appl Microbiol. 2009 Feb;48(2):230-3. doi: 10.1111/j.1472-765X.2008.02517.x.
This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples.
A total of 285 stool samples were inoculated onto Chromogenic VRE Agar (AES VRE agar; AES Chemunex), chromID VRE (bioMérieux) and VRE Agar (Oxoid) both directly and also following broth enrichment. In total 18 strains of vancomycin-resistant Enterococcus faecium were recovered, including 17 harbouring the vanA gene and one with vanB. On direct culture, the sensitivity of the three media was 66.7%, 77.8% and 44.4% and after broth enrichment 66.7%, 83.3% and 77.8% using AES VRE Agar, chromID VRE and Oxoid VRE Agar respectively.
All three media are useful tools for the isolation of VRE from stool samples. AES VRE Agar and bioMérieux chromID VRE are easier to use than Oxoid VRE Agar due to diffusion of black coloration from the latter.
This is the first study to evaluate the performance of AES VRE Agar and the first to compare two media containing synthetic chromogens for the isolation of VRE.
本研究旨在评估两种用于分离耐万古霉素肠球菌（VRE）的显色培养基的性能，并将它们与传统的胆汁七叶苷培养基进行比较，以从粪便样本中分离VRE。
总共285份粪便样本直接接种以及肉汤增菌后接种到显色VRE琼脂（AES VRE琼脂；AES Chemunex）、chromID VRE（生物梅里埃公司）和VRE琼脂（奥克托公司）上。总共分离出18株耐万古霉素屎肠球菌，其中17株携带vanA基因，1株携带vanB基因。直接培养时，三种培养基的敏感性分别为66.7%、77.8%和44.4%，肉汤增菌后，使用AES VRE琼脂、chromID VRE和奥克托公司VRE琼脂的敏感性分别为66.7%、83.3%和77.8%。
所有三种培养基都是从粪便样本中分离VRE的有用工具。由于奥克托公司VRE琼脂会出现黑色扩散现象，AES VRE琼脂和生物梅里埃公司的chromID VRE比其更易于使用。
这是第一项评估AES VRE琼脂性能的研究，也是第一项比较两种含合成色原的培养基用于分离VRE的研究。
