Department of Chemical and Biochemical Engineering, Xiamen University, Xiamen 361005, China.
Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan.
Sensors (Basel). 2018 Jun 5;18(6):1823. doi: 10.3390/s18061823.
In this study, we provided the detailed characterizations of our recent HRP-AuNPs/PEDOT:BSA/Pt biosensor, constructed through a simple fabrication procedure with improved stability and good sensitivity. Raman and Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy not only confirmed the synthesis of conductive PEDOT where BSA was the template for the polymerization, but also provided further insights into the stable immobilization of AuNP on the PEDOT:BSA film. Scanning electron microscopy revealed that the attachment of AuNPs were stable under a high salt environment. The current technology demonstrates a feasible procedure to form a functional AuNPs/PEDOT:BSA film that has potential applications in the fabrication of various biosensors and electric devices.
在这项研究中，我们详细描述了我们最近研发的 HRP-AuNPs/PEDOT:BSA/Pt 生物传感器，该传感器通过简单的制造工艺构建，具有改进的稳定性和良好的灵敏度。拉曼和傅里叶变换红外光谱以及 X 射线光电子能谱不仅证实了导电 PEDOT 的合成，其中 BSA 是聚合的模板，而且还提供了对 AuNP 在 PEDOT:BSA 薄膜上稳定固定的进一步了解。扫描电子显微镜显示，AuNPs 的附着在高盐环境下也很稳定。这项技术展示了一种可行的方法，可以形成功能性的 AuNPs/PEDOT:BSA 薄膜，该薄膜在制造各种生物传感器和电子设备方面具有潜在应用。
