Bulat S A, Mikhaĭlo N V, Koroliuk A M
Zh Mikrobiol Epidemiol Immunobiol. 1991 Dec(12):2-7.
Genetic analysis of 19 standard strains belonging to 6 Yersinia species (Y. pestis, Y. pseudotuberculosis, Y. enterocolitica, Y. kirstensenii, Y. frederiksenii, Y. intermedia) revealed that gene typing by the method of polymerase chain reaction (PCR) with the use of universal primers permitted the identification of species in bacterial cultures by PCR patterns and the determination of Y. pseudotuberculosis serovars within 4 hours. By this method 23 Y. pseudotuberculosis strains (serovar 1), earlier isolated in different regions of the USSR from humans and rodents, were studied. The study showed that out of 14 strains of human origin only two strains could actually be classified with serovar 1, while the remaining strains were reidentified as belonging to serovar 5. Among 9 strains isolated from rodents those of serovar 1 prevailed (8 strains). The authors suppose that strains of serovar 5 cause outbreaks and sporadic cases of pseudotuberculosis, occurring considerably more often than it is commonly believed in the USSR.
对属于6种耶尔森氏菌（鼠疫耶尔森氏菌、假结核耶尔森氏菌、小肠结肠炎耶尔森氏菌、克氏耶尔森氏菌、弗氏耶尔森氏菌、中间型耶尔森氏菌）的19个标准菌株进行的基因分析表明，使用通用引物通过聚合酶链反应（PCR）方法进行基因分型，可以通过PCR图谱鉴定细菌培养物中的菌种，并在4小时内确定假结核耶尔森氏菌的血清型。通过这种方法，对23株先前在苏联不同地区从人和啮齿动物中分离出的假结核耶尔森氏菌菌株（血清型1）进行了研究。研究表明，在14株人类来源的菌株中，实际上只有两株可归类为血清型1，而其余菌株被重新鉴定为属于血清型5。在从啮齿动物中分离出的9株菌株中，血清型1的菌株占主导（8株）。作者推测，血清型5的菌株会引发假结核的暴发和散发病例，其发生频率比苏联普遍认为的要高得多。
