Scott Julie C, Koylass Mark S, Stubberfield Michael R, Whatmore Adrian M
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom.
Appl Environ Microbiol. 2007 Nov;73(22):7331-7. doi: 10.1128/AEM.00976-07. Epub 2007 Sep 21.
The genus Brucella includes a number of species that are major animal pathogens worldwide and significant causes of zoonotic infections of humans. Traditional methods of identifying Brucella to the species level can be time-consuming, can be subjective, and can pose a hazard to laboratory personnel in the absence of suitable biocontainment facilities. Using a robust phylogenetic framework, a number of single-nucleotide polymorphisms (SNPs) that define particular species within the genus were identified. These SNPs were used to develop a multiplex SNP detection assay, based on primer extension technology, that can rapidly and unambiguously identify an isolate as a member of one of the six classical Brucella species or as a member of the recently identified marine mammal group.
布鲁氏菌属包含多个物种，这些物种是全球主要的动物病原体，也是人类人畜共患感染的重要病因。将布鲁氏菌鉴定到种水平的传统方法可能耗时、主观，并且在缺乏合适生物安全防护设施的情况下会对实验室人员构成危害。利用一个强大的系统发育框架，确定了一些能界定该属内特定物种的单核苷酸多态性（SNP）。这些SNP被用于开发一种基于引物延伸技术的多重SNP检测方法，该方法能够快速、明确地将分离株鉴定为六种经典布鲁氏菌物种之一的成员，或者鉴定为最近确定的海洋哺乳动物组的成员。
