Di Dongdong, Cui Buyun, Wang Heng, Zhao Hongyan, Piao Dongri, Tian Lili, Tian Guozhong, Kang Jingli, Mao Xiang, Zhang Xiaojun, Du Pengfei, Zhu Lin, Zhao Zhuo, Mao Lingling, Yao Wenqing, Guan Pingyuan, Fan Weixing, Jiang Hai
Laboratory of Zoonoses, China Animal Health and Epidemiology Center, MOA, Qingdao, China.
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
PLoS One. 2014 Jan 23;9(1):e84862. doi: 10.1371/journal.pone.0084862. eCollection 2014.
In China, brucellosis is an endemic disease typically caused by Brucella melitensis infection (biovars 1 and 3). Brucella canis infection in dogs has not traditionally recognized as a major problem. In recent years however, brucellosis resulting from Brucella canis infection has also been reported, suggesting that infections from this species may be increasing. Data concerning the epidemiology of brucellosis resulting from Brucella canis infection is limited. Therefore, the purpose of this study was to assess the diversity among Chinese Brucella canis strains for epidemiological purposes. First, we employed a 16-marker VNTR assay (Brucella MLVA-16) to assess the diversity and epidemiological relationship of 29 Brucella canis isolates from diverse locations throughout China with 38 isolates from other countries. MLVA-16 analysis separated the 67 Brucella canis isolates into 57 genotypes that grouped into five clusters with genetic similarity coefficients ranging from 67.73 to 100%. Moreover, this analysis revealed a new genotype (2-3-9-11-3-1-5-1:118), which was present in two isolates recovered from Guangxi in 1986 and 1987. Second, multiplex PCR and sequencing analysis were used to determine whether the 29 Chinese Brucella canis isolates had the characteristic BMEI1435 gene deletion. Only two isolates had this deletion. Third, amplification of the omp25 gene revealed that 26 isolates from China had a T545C mutation. Collectively, this study reveals that considerable diversity exists among Brucella canis isolates in China and provides resources for studying the genetic variation and microevolution of Brucella.
在中国，布鲁氏菌病是一种地方病，通常由羊种布鲁氏菌感染（生物变种1和3）引起。传统上，犬种布鲁氏菌感染在犬类中不被视为主要问题。然而，近年来，也有报道称犬种布鲁氏菌感染导致的布鲁氏菌病，这表明该物种的感染可能在增加。关于犬种布鲁氏菌感染导致的布鲁氏菌病流行病学的数据有限。因此，本研究的目的是评估中国犬种布鲁氏菌菌株之间的多样性，以用于流行病学研究。首先，我们采用了一种16标记的可变数目串联重复序列分析（布鲁氏菌多位点可变数目串联重复序列分析-16，Brucella MLVA-16）来评估来自中国各地不同地点的29株犬种布鲁氏菌分离株与来自其他国家的38株分离株之间的多样性和流行病学关系。MLVA-16分析将67株犬种布鲁氏菌分离株分为57个基因型，这些基因型聚为五个簇，遗传相似系数范围为67.73%至100%。此外，该分析还揭示了一种新的基因型（2-3-9-11-3-1-5-1:118），它存在于1986年和1987年从广西分离出的两株菌株中。其次，采用多重聚合酶链反应和测序分析来确定29株中国犬种布鲁氏菌分离株是否具有特征性的BMEI1435基因缺失。只有两株分离株有这种缺失。第三，omp25基因的扩增显示，来自中国的26株分离株有一个T545C突变。总体而言，本研究表明中国犬种布鲁氏菌分离株之间存在相当大的多样性，并为研究布鲁氏菌的遗传变异和微观进化提供了资源。
