Harrow Sally A, Ravindran Velmurugu, Butler Ruth C, Marshall John W, Tannock Gerald W
New Zealand Institute for Crop & Food Research Limited, Christchurch, New Zealand.
Appl Environ Microbiol. 2007 Nov;73(22):7123-7. doi: 10.1128/AEM.01289-07. Epub 2007 Sep 21.
A real-time quantitative PCR assay targeting a 16S-23S intergenic spacer region sequence was devised to measure the sizes of populations of Lactobacillus salivarius present in ileal digesta collected from broiler chickens. This species has been associated with deconjugation of bile salts in the small bowel and reduced broiler productivity. The assay was tested as a means of monitoring the sizes of L. salivarius populations from broilers fed diets with different compositions, maintained at different stocking densities, or given the antimicrobial drugs bacitracin and monensin in the feed. Stocking densities did not influence the numbers of L. salivarius cells in the ileum. A diet containing meat and bone meal reduced the size of the L. salivarius population relative to that of chickens given the control diet, as did administration of bacitracin and monensin in the feed. These changes in the target bacterial population were associated with improved broiler weight gain.
设计了一种针对16S - 23S基因间隔区序列的实时定量PCR检测方法，以测量从肉鸡回肠消化物中提取的唾液乳杆菌群体数量。该菌种与小肠中胆汁盐的去结合作用以及肉鸡生产性能下降有关。该检测方法作为一种监测手段，用于检测不同日粮组成、不同饲养密度或在饲料中添加抗菌药物杆菌肽和莫能菌素的肉鸡中唾液乳杆菌群体数量。饲养密度不影响回肠中唾液乳杆菌细胞数量。与饲喂对照日粮的鸡相比，含有肉骨粉的日粮会使唾液乳杆菌群体数量减少，饲料中添加杆菌肽和莫能菌素也会导致同样的结果。目标细菌群体的这些变化与肉鸡体重增加改善有关。
