Gong J, Yu H, Liu T, Gill J J, Chambers J R, Wheatcroft R, Sabour P M
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
J Appl Microbiol. 2008 May;104(5):1372-82. doi: 10.1111/j.1365-2672.2007.03699.x. Epub 2008 Jan 14.
Determining the effects of zinc bacitracin, bird age and access to range on bacterial microbiota in the ileum and caeca of broilers.
16S rRNA gene-based polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) profiling, DNA sequencing and real-time quantitative PCR techniques were used. The richness of both ileal and caecal microbiota increased with chicken age. The microbiota from those birds of the same age demonstrated relatively similar PCR-DGGE profiles and tended to form closely related clusters in the relatedness analyses. Dietary treatment with bacitracin (50 mg kg(-1)) and access to range did not change the richness but altered the composition of the microbiota. The impact of bacitracin was particularly obvious in 3-day-old chicks. Lactobacilli were abundant in the caecal microbiota of 3-day-old chicks regardless of the dietary treatment with bacitracin. The access to range enriched Bifidobacterium in both the ileum and caeca.
Bacitracin, bird age and access to range all influenced bacterial microbiota in the ileum and caeca of broilers, with bird age having the greatest apparent effect.
Providing useful information for the development of antibiotic replacement therapy for poultry production.
确定杆菌肽锌、鸡龄和户外活动对肉鸡回肠和盲肠细菌微生物群的影响。
采用基于16S rRNA基因的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析、DNA测序和实时定量PCR技术。回肠和盲肠微生物群的丰富度均随鸡龄增加而增加。相同鸡龄的鸡的微生物群表现出相对相似的PCR-DGGE图谱，并且在相关性分析中倾向于形成密切相关的聚类。杆菌肽（50 mg kg(-1)）饮食处理和户外活动并没有改变微生物群的丰富度，但改变了其组成。杆菌肽对3日龄雏鸡的影响尤为明显。无论是否用杆菌肽进行饮食处理，3日龄雏鸡盲肠微生物群中乳酸杆菌都很丰富。户外活动使回肠和盲肠中的双歧杆菌增多。
杆菌肽、鸡龄和户外活动均影响肉鸡回肠和盲肠的细菌微生物群，其中鸡龄的影响最为明显。
为家禽生产中抗生素替代疗法的开发提供有用信息。
