Strozycki Pawel M, Szczurek Anna, Lotocka Barbara, Figlerowicz Marek, Legocki Andrzej B
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
J Exp Bot. 2007;58(12):3145-53. doi: 10.1093/jxb/erm152. Epub 2007 Sep 22.
An ability to form symbiotic associations with rhizobia and to utilize atmospheric nitrogen makes legumes ecologically successful. High iron content in legume grains, partially relocated from root nodules, is another-nutritional-advantage of this group of plants. The ferritin complex is the major cell iron storage and detoxification unit and has been recognized as a marker of many stress-induced responses. The possible participation of ferritin in nodule formation and functioning was investigated here. Correlation of increased accumulation of both ferritin polypeptide and mRNA with actual in situ localization of ferritin allowed ferritin synthesis in the developing, indeterminate-type root nodules to be related to differentiating bacteroid tissue. This kind of tissue, in contrast to the determinate-type nodules, is present in lupin nodules at almost all stages of their development. Interestingly, it was found that, in this type of nodule, senescence starting in the decaying zones induces ferritin accumulation in younger, still active, tissues. Based on the presented data, and in correlation with previous results, some aspects of the regulation of expression of lupin ferritin genes are also discussed.
与根瘤菌形成共生关系并利用大气氮的能力使豆科植物在生态上获得成功。豆科植物种子中高铁含量（部分从根瘤重新分配而来）是这类植物的另一个营养优势。铁蛋白复合体是细胞内主要的铁储存和解毒单位，并且已被视为许多应激诱导反应的标志物。本文研究了铁蛋白在根瘤形成和功能发挥过程中可能的参与情况。铁蛋白多肽和mRNA积累的增加与铁蛋白实际原位定位之间的相关性，使得发育中的不定型根瘤中铁蛋白的合成与正在分化的类菌体组织相关联。与定型根瘤不同，这种组织在羽扇豆根瘤发育的几乎所有阶段都存在。有趣的是，研究发现，在这种类型的根瘤中，从腐烂区域开始的衰老过程会诱导铁蛋白在较年轻且仍活跃的组织中积累。基于所呈现的数据，并与先前的结果相关联，还讨论了羽扇豆铁蛋白基因表达调控的一些方面。
