Monstein H-J, Truedsson M, Ryberg A, Ohlsson B
Clinical Microbiology, Molecular Biology Laboratory-LMC, University Hospital, Linköping, Sweden.
Eur Surg Res. 2008;40(1):34-40. doi: 10.1159/000108655. Epub 2007 Sep 21.
BACKGROUND/AIM: Vasopressin and oxytocin are closely related peptides, and both exert effects on the gastrointestinal function. In the present study, we wanted to map the expression of vasopressin receptor mRNAs (V1a, V1b/V3, and V2) in nontumorous tissue biopsy specimens of human gastrointestinal tract and surrounding tissues.
Total and polyA+ RNAs were isolated from human tissue biopsy specimens using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA. Semi-nested PCR amplifications were carried out, using gene-specific V1a, V1b/V3, and V2 receptor primers. The PCR amplicons were partially sequenced to confirm their identity.
The present study demonstrated the expression of vasopressin receptor mRNAs in human gastrointestinal tract, pancreas, kidney, lung, brain, and ovary. The expression pattern varied between different parts of the gastrointestinal tract. In the colon ascendens, V1a receptor mRNA expression could not be detected in 3 out of 4 analyzed tissue biopsy specimens. On the other hand, all the vasopressin receptor mRNAs were expressed in all colon transversum biopsy samples.
V1a, V1b/V3, and V2 receptor mRNAs are widely expressed throughout human gastrointestinal tract and surrounding tissues. The data obtained provide information for further mapping and determination of the physiological role of the vasopressin receptor mRNA expression in normal and tumorous tissues.
背景/目的：血管加压素和催产素是密切相关的肽类，二者均对胃肠功能产生影响。在本研究中，我们旨在描绘血管加压素受体mRNA（V1a、V1b/V3和V2）在人胃肠道及周围组织的非肿瘤组织活检标本中的表达情况。
使用自动核酸提取仪从人组织活检标本中分离总RNA和聚腺苷酸加尾RNA（polyA+ RNA），随后将其转化为单链cDNA。使用基因特异性的V1a、V1b/V3和V2受体引物进行半巢式PCR扩增。对PCR扩增产物进行部分测序以确认其身份。
本研究证实血管加压素受体mRNA在人胃肠道、胰腺、肾脏、肺、脑和卵巢中均有表达。胃肠道不同部位的表达模式有所不同。在升结肠中，4份分析的组织活检标本中有3份未检测到V1a受体mRNA表达。另一方面，所有横结肠活检样本中均表达了所有血管加压素受体mRNA。
V1a、V1b/V3和V2受体mRNA在整个人胃肠道及周围组织中广泛表达。所获得的数据为进一步描绘和确定血管加压素受体mRNA在正常和肿瘤组织中的生理作用提供了信息。
