Mortensen P E, Olsen J, Bülow J, Hage E, Edelfors S
Rigshospitalet, Department of Medical Physiology B, University of Copenhagen, Denmark.
Acta Physiol Scand Suppl. 1991;603:75-84.
A 133Xe washout technique for measuring the blood flow in the intestinal mucosa is introduced and evaluated. In 11 anaesthetized pigs a laparotomy was performed and the mucosal blood flow rate in the intestine of the pig was determined by a local epimucosal application of 133Xe. In both the colon and the small intestine the 133Xe washout plotted in a semilogarithmic diagram showed a multiexponential configuration. Histological examination and localization studies showed shunting by diffusion of 133Xe in the intestinal mucosa explaining the multiexponential configuration of the washout curve. Therefore the initial slope of the washout was used for measuring blood flow rate. Blood flow rate was simultaneously measured by microsphere entrapment technique. There was an excellent correlation between the blood flow rate determined by the two techniques the correlation coefficient R being 0.89 in the small intestine and 0.996 in the colon.
本文介绍并评估了一种用于测量肠黏膜血流量的¹³³Xe洗脱技术。对11只麻醉猪进行剖腹手术，通过在猪肠黏膜局部应用¹³³Xe来测定肠黏膜血流速率。在结肠和小肠中，¹³³Xe洗脱在半对数图上均呈现多指数形态。组织学检查和定位研究表明，¹³³Xe在肠黏膜中通过扩散发生分流，这解释了洗脱曲线的多指数形态。因此，洗脱的初始斜率用于测量血流速率。同时采用微球捕获技术测量血流速率。两种技术测定的血流速率之间具有极好的相关性，小肠中的相关系数R为0.89，结肠中的相关系数R为0.996。
