Salzmann C C, Cozzolino S, Schiestl F P
Plant Ecological Genetics, Institute of Integrative Biology, ETH Zürich, Universitätsstrasse 16, 8092 Zurich, Switzerland.
Plant Biol (Stuttg). 2007 Nov;9(6):720-9. doi: 10.1055/s-2007-965614. Epub 2007 Sep 21.
One third of all orchid species are deceptive and do not reward their pollinators. Such deceptive orchids are often characterised by unusually high variation in floral signals such as colour and scent. In this study, we investigated the scent composition of two Mediterranean food-deceptive orchids Orchis mascula, Orchis pauciflora, and their hybrid, O. x colemanii. Scent was collected IN SITU by headspace sorption and was subsequently analysed with gas chromatography and gas chromatography-mass spectrometry. We compared variation of odour compounds within and between populations as well as species. We identified 35 floral scent compounds, mainly monoterpenes, which were shared by both species. Both quantitative and qualitative variability within and among populations was high. Many individuals within species could be classified to different "odour-types". In spite of high qualitative and quantitative intra- and inter-population variability, the species were clearly differentiated in their scent bouquets, whereas most hybrid individuals emitted an intermediate scent.
所有兰花物种中有三分之一具有欺骗性，不会对传粉者给予回报。这类具有欺骗性的兰花通常具有花朵信号（如颜色和气味）异常高的变异性。在本研究中，我们调查了两种地中海食源性欺骗性兰花——雄红门兰、少花红门兰及其杂交种科尔曼红门兰的气味成分。通过顶空吸附原位采集气味，随后用气相色谱和气相色谱 - 质谱联用仪进行分析。我们比较了种群内部和种群之间以及物种之间气味化合物的变异性。我们鉴定出35种花朵气味化合物，主要是单萜类化合物，这两个物种都有这些化合物。种群内部和种群之间在数量和质量上的变异性都很高。物种内的许多个体可以被归类为不同的“气味类型”。尽管种群内部和种群之间在质量和数量上存在高变异性，但这些物种在其气味组合上有明显差异，而大多数杂交个体散发出的是中间气味。
